• Basic information
• Learning outcomes
• Contents
• Assessment
• Workload

Learning outcomes

Upon successful completion of this course, students will be able to:

1. Identify unknown bacteria by using microbiological methods

   Contribution to Program Outcomes

   1. To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
   2. To be able to explain the genetic information flow in organisms and populations.
   3. To be able to work individually, make independent decisions and participate actively in multidisciplinary group studies.

   Method of assessment

   1. Written exam
   2. Laboratory exercise/exam
2. Prepare pure culture by using aseptic techniques

   Contribution to Program Outcomes

   1. To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
   2. To be able to work individually, make independent decisions and participate actively in multidisciplinary group studies.
   3. To be able to apply biological concepts to individual, social, economic, technologic and environmental issues and to develop sustainable approaches for problem solving.

   Method of assessment

   1. Written exam
   2. Laboratory exercise/exam
3. Know how to control microorganisms by using physical and chemical agents

   Contribution to Program Outcomes

   1. To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
   2. To be able to work individually, make independent decisions and participate actively in multidisciplinary group studies.
   3. To be able to apply biological concepts to individual, social, economic, technologic and environmental issues and to develop sustainable approaches for problem solving.

   Method of assessment

   1. Written exam
   2. Laboratory exercise/exam
4. Prepare media to differentiate between bacteria

   Contribution to Program Outcomes

   1. To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
   2. To be able to work individually, make independent decisions and participate actively in multidisciplinary group studies.
   3. To be able to apply biological concepts to individual, social, economic, technologic and environmental issues and to develop sustainable approaches for problem solving.

   Method of assessment

   1. Written exam
   2. Laboratory exercise/exam
5. Gain knowledge about properties of microorganisms

   Contribution to Program Outcomes

   1. To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
   2. To be able to understand the relationship between matter and energy in organisms.
   3. To be able to explain the genetic information flow in organisms and populations.
   4. To be able to work individually, make independent decisions and participate actively in multidisciplinary group studies.
   5. To be able to apply biological concepts to individual, social, economic, technologic and environmental issues and to develop sustainable approaches for problem solving.

   Method of assessment

   1. Written exam
   2. Laboratory exercise/exam

Contents
Week 1:	General safety rules in microbiology laboratory
Week 2:	Preparation of bacteriological media and different sterilization techniques
Week 3:	Aseptic techniques, techniques for pure culture preparation
Week 4:	Sample preparation for microscopic examination and staining techniques I (Smear 5-preparation from liquid and solid cultures, Simple staining, Negative staining)
Week 5:	Sample preparation for microscopic examination and staining techniques II (Smear preparation from liquid and solid cultures, Endospore staining, Gram staining, Capsule staining)
Week 6:	Bacterial growth on differential media (hemolysis in blood agar, growth of Gr (+), Gr (-) and patogenic bacteria on special media)
Week 7:	MIDTERM Introduction of Biochemical activities of bacteria
Week 8:	Biochemical activities of bacteria I (Carbohydrate metabolism) (Carbohydrate test, Starch test, TSI agar)
Week 9:	Biochemical activities of bacteria II (Nitrogen metabolism) (Denitrification test, Protein utilization test, Decarboxylase test, Gelatin test, Urea utilization test)
Week 10:	Biochemical activities of bacteria III (Catalase test, Oxidase test, Thioglicolate test)
Week 11:	Biochemical activities of bacteria IV (Indol test, Voges Proskauer test, Metyl red test, Citrate test)
Week 12:	Control of microorganisms with chemical factors I (Antibiotic resistance test)
Week 13:	Control of microorganisms with chemical factors II (Evaluation of antiseptics, Heavy metals and other chemicals)
Week 14:	Serial dilution and viable cell count Evaluation of results of viable cell count, calculating CFU (colony forming units)
Week 15*:	------
Week 16*:	FINAL EXAM
Textbooks and materials:	Laboratuvar notları (kitapçık)
Recommended readings:	[1] Madigan, M.T., Martinko, J.M., Bender, K.S., Buckley, D. H., Stahl, D.A. 15th Ed. Brock Biology of Microorganisims. Pearson Education Inc. San Francisco [2] Cappuccino James, Sherman Natalie-Microbiology. A Laboratory Manual-Tenth Edition-Pearson Education
	* Between 15th and 16th weeks is there a free week for students to prepare for final exam.

Assessment
Method of assessment	Week number	Weight (%)
Mid-terms:	7	25
Other in-term studies:		0
Project:	16	25
Homework:		0
Quiz:	4,5,6,8,9,10,11,12,13,14	20
Final exam:	16	30
	Total weight:	(%)

Workload
Activity	Duration (Hours per week)	Total number of weeks	Total hours in term
Courses (Face-to-face teaching):	2	14
Own studies outside class:	2	6
Practice, Recitation:	0	0
Homework:	1	13
Term project:	0	0
Term project presentation:	0	0
Quiz:	0	0
Own study for mid-term exam:	3	3
Mid-term:	1	1
Personal studies for final exam:	3	3
Final exam:	2	1
		Total workload:
		Total ECTS credits:	*
	* ECTS credit is calculated by dividing total workload by 25. (1 ECTS = 25 work hours)