Syllabus ( MBG 307 )
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Basic information
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| Course title: |
Biochemistry Laboratory |
| Course code: |
MBG 307 |
| Lecturer: |
Assoc. Prof. Dr. F. İnci ÖZDEMİR
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| ECTS credits: |
5 |
| GTU credits: |
2 (0+2+0) |
| Year, Semester: |
3, Fall |
| Level of course: |
First Cycle (Undergraduate) |
| Type of course: |
Compulsory
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| Language of instruction: |
English
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| Mode of delivery: |
Face to face , Lab work
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| Pre- and co-requisites: |
None |
| Professional practice: |
No |
| Purpose of the course: |
Teach how to purify proteins by chromatographic methods; the characterization of the purified enzymes; the enzyme kinetics parameters; the biochemical methods like spectroscopy, colon chromatography, gel filtration chromatography, SDS PAGE, Western Blot etc.
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Learning outcomes
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Upon successful completion of this course, students will be able to:
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Apply chromatographic methods for protein purifications
Contribution to Program Outcomes
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To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
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To be able to apply biological concepts to individual, social, economic, technologic and environmental issues and to develop sustainable approaches for problem solving.
Method of assessment
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Laboratory exercise/exam
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Characterize the purified enzymes
Contribution to Program Outcomes
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To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
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To be able to work individually, make independent decisions and participate actively in multidisciplinary group studies.
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To be able to drive hypotheses using existing knowledge, designing and conducting experiment for problem solving and make correct interpretation of the results obtained from the experiment.
Method of assessment
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Laboratory exercise/exam
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Apply spectroscopy, colon chromatography, gel filtration chromatography, SDS PAGE, Western Blot for various protein works.
Contribution to Program Outcomes
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To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
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To be able to drive hypotheses using existing knowledge, designing and conducting experiment for problem solving and make correct interpretation of the results obtained from the experiment.
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To be able to apply biological concepts to individual, social, economic, technologic and environmental issues and to develop sustainable approaches for problem solving.
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To be able to embrace academic ethical rules and to be able to act with a sense of responsibility.
Method of assessment
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Laboratory exercise/exam
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Apply different methodologies for studying the enzyme kinetics
Contribution to Program Outcomes
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To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
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To be able to drive hypotheses using existing knowledge, designing and conducting experiment for problem solving and make correct interpretation of the results obtained from the experiment.
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To be able to apply biological concepts to individual, social, economic, technologic and environmental issues and to develop sustainable approaches for problem solving.
Method of assessment
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Laboratory exercise/exam
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Contents
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| Week 1: |
Introduction
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| Week 2: |
Calculations |
| Week 3: |
Buffers |
| Week 4: |
Determination oF The pKA Values of an Amino Acid |
| Week 5: |
Colorimetry / Spectrophotometry |
| Week 6: |
Determination of Protein Concentration (Bradford Protein Assay) |
| Week 7: |
Repeat
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| Week 8: |
Midterm Exam
Western Blot
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| Week 9: |
Protein Precipitation by Salts |
| Week 10: |
Color Reactions and Reducing Sugar Reactions of Carbohydrates |
| Week 11: |
Gel Filtration Chromatography I |
| Week 12: |
Gel Filtration Chromatography II |
| Week 13: |
Kinetics of ß-Fructofuranosidase (Part 1)
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| Week 14: |
Kinetics of ß-Fructofuranosidase (Part 2)
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| Week 15*: |
Tekrar |
| Week 16*: |
Final Exam
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| Textbooks and materials: |
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| Recommended readings: |
Laboratuvar Kitapçığı dersin hocaları tarafından hazırlanacak ve dağıtılacaktır
To be announced |
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* Between 15th and 16th weeks is there a free week for students to prepare for final exam.
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Assessment
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| Method of assessment |
Week number |
Weight (%) |
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| Mid-terms: |
8 |
30 |
| Other in-term studies: |
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0 |
| Project: |
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0 |
| Homework: |
2-14 |
15 |
| Quiz: |
2-14 |
15 |
| Final exam: |
16 |
40 |
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Total weight: |
(%) |
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Workload
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| Activity |
Duration (Hours per week) |
Total number of weeks |
Total hours in term |
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| Courses (Face-to-face teaching): |
1 |
14 |
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| Own studies outside class: |
2 |
14 |
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| Practice, Recitation: |
2 |
14 |
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| Homework: |
2 |
14 |
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| Term project: |
0 |
0 |
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| Term project presentation: |
0 |
0 |
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| Quiz: |
1 |
14 |
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| Own study for mid-term exam: |
1 |
14 |
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| Mid-term: |
2 |
1 |
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| Personal studies for final exam: |
0 |
0 |
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| Final exam: |
2 |
1 |
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Total workload: |
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Total ECTS credits: |
* |
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* ECTS credit is calculated by dividing total workload by 25.
(1 ECTS = 25 work hours)
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