Syllabus ( MBG 326 )
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Basic information
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| Course title: |
Molecular Genetics Laboratory |
| Course code: |
MBG 326 |
| Lecturer: |
Assist. Prof. Saliha İŞSEVER ÖZTÜRK
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| ECTS credits: |
5 |
| GTU credits: |
2 (0+4+0) |
| Year, Semester: |
3, Spring |
| Level of course: |
First Cycle (Undergraduate) |
| Type of course: |
Compulsory
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| Language of instruction: |
English
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| Mode of delivery: |
Face to face , Group study , Lab work
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| Pre- and co-requisites: |
MBG348 Molecular Biotechnology |
| Professional practice: |
No |
| Purpose of the course: |
In this course, fundamental recombinant DNA techniques used in molecular biology will be handled experimentally. |
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Learning outcomes
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Upon successful completion of this course, students will be able to:
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Recall main recombinant DNA techniques.
Contribution to Program Outcomes
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To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
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To be able to work individually, make independent decisions and participate actively in multidisciplinary group studies.
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To be able to apply biological concepts to individual, social, economic, technologic and environmental issues and to develop sustainable approaches for problem solving.
Method of assessment
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Written exam
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Laboratory exercise/exam
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Do molecular cloning.
Contribution to Program Outcomes
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To be able to work individually, make independent decisions and participate actively in multidisciplinary group studies.
Method of assessment
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Written exam
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Laboratory exercise/exam
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Analyse gene products belonging to his/her clones.
Contribution to Program Outcomes
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To be able to work individually, make independent decisions and participate actively in multidisciplinary group studies.
Method of assessment
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Written exam
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Laboratory exercise/exam
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Gain knowledge about working safely in a molecular biology laboratory.
Contribution to Program Outcomes
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To be able to embrace academic ethical rules and to be able to act with a sense of responsibility.
Method of assessment
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Laboratory exercise/exam
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Gain knowledge about interpreting and reporting data.
Contribution to Program Outcomes
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To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
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To be able to work individually, make independent decisions and participate actively in multidisciplinary group studies.
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To be able to drive hypotheses using existing knowledge, designing and conducting experiment for problem solving and make correct interpretation of the results obtained from the experiment.
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To be able to embrace academic ethical rules and to be able to act with a sense of responsibility.
Method of assessment
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Homework assignment
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Contents
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| Week 1: |
Working and safety rules in a molecular biology laboratory |
| Week 2: |
Bacterial genomic DNA isolation and Spectrophotometric analysis of DNA |
| Week 3: |
Gene amplification via PCR |
| Week 4: |
Restriction enzyme digestion |
| Week 5: |
Agarose gel elctrophoresis and DNA isolation from agarose gel |
| Week 6: |
Molecular cloning |
| Week 7: |
Preparation of bacterial competent cells |
| Week 8: |
Bacterial transformation |
| Week 9: |
Colony Selection Techniques: Blue-white colony screening |
| Week 10: |
Miniprep plasmid DNA isolation |
| Week 11: |
Restriction mapping |
| Week 12: |
Recombinant gene expression |
| Week 13: |
SDS/PAGE analysis |
| Week 14: |
Enzymatic activity assays |
| Week 15*: |
- |
| Week 16*: |
Final Exam |
| Textbooks and materials: |
Protocol for each experiment is delivered weekly to the students. |
| Recommended readings: |
1. Ausubel, F. M., et. al., (2004), "Current protocols in Molecular Biology", J. Wiley and Sons.
2. Sambrook, J., Fritsch, E.F., Maniatis T., (1989), "Molecular Cloning: a Laboratory Manual", 2nd Ed., Cold Spring Harbor Laboratory Press.
3. Stephenson, F. H. (2003), "Calculations in Molecular Biology and Biotechnology: a Guide to Mathematics in the Laboratory", Academic Press.
4. Brown T. A., (2016), "Gene Cloning and DNA Analysis: An Introduction", 7th Ed., Blackwell Publishing.
5. Wink, M., (2006), "An Introduction to Molecular Biotechnology: Molecular Fundamentals, Methods and Applications in Modern Biotechnology", Wiley-VCH.
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* Between 15th and 16th weeks is there a free week for students to prepare for final exam.
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Assessment
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| Method of assessment |
Week number |
Weight (%) |
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| Mid-terms: |
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0 |
| Other in-term studies: |
2-14 |
25 |
| Project: |
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0 |
| Homework: |
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0 |
| Quiz: |
2-14 |
25 |
| Final exam: |
16 |
50 |
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Total weight: |
(%) |
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Workload
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| Activity |
Duration (Hours per week) |
Total number of weeks |
Total hours in term |
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| Courses (Face-to-face teaching): |
1 |
14 |
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| Own studies outside class: |
4 |
13 |
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| Practice, Recitation: |
3 |
14 |
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| Homework: |
0 |
0 |
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| Term project: |
0 |
0 |
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| Term project presentation: |
0 |
0 |
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| Quiz: |
0.5 |
13 |
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| Own study for mid-term exam: |
0 |
0 |
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| Mid-term: |
0 |
0 |
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| Personal studies for final exam: |
10 |
1 |
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| Final exam: |
2 |
1 |
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Total workload: |
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Total ECTS credits: |
* |
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* ECTS credit is calculated by dividing total workload by 25.
(1 ECTS = 25 work hours)
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