Syllabus ( MBG 333 )
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Basic information
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| Course title: |
Molecular Cell Biology Laboratory |
| Course code: |
MBG 333 |
| Lecturer: |
Prof. Dr. Asuman DEMİROĞLU-ZERGEROĞLU
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| ECTS credits: |
5 |
| GTU credits: |
2 (0+4+0) |
| Year, Semester: |
3, Spring |
| Level of course: |
First Cycle (Undergraduate) |
| Type of course: |
Compulsory
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| Language of instruction: |
English
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| Mode of delivery: |
Face to face , Group study , Lab work
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| Pre- and co-requisites: |
None |
| Professional practice: |
No |
| Purpose of the course: |
The students will be taught the principles of molecular cell biology methodology as well gain to interpret and analyze the scientific data from specific experiments
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Learning outcomes
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Upon successful completion of this course, students will be able to:
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Comprehend and describe the principles of molecular cell biology methodology
Contribution to Program Outcomes
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To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
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To be able to drive hypotheses using existing knowledge, designing and conducting experiment for problem solving and make correct interpretation of the results obtained from the experiment.
Method of assessment
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Written exam
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Laboratory exercise/exam
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Interpret and analyze the scientific data from specific experiments
Contribution to Program Outcomes
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To be able to drive hypotheses using existing knowledge, designing and conducting experiment for problem solving and make correct interpretation of the results obtained from the experiment.
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To be able to embrace academic ethical rules and to be able to act with a sense of responsibility.
Method of assessment
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Written exam
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Laboratory exercise/exam
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Working as an individual or as in a team
Contribution to Program Outcomes
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To be able to work individually, make independent decisions and participate actively in multidisciplinary group studies.
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To be able to embrace academic ethical rules and to be able to act with a sense of responsibility.
Method of assessment
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Written exam
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Laboratory exercise/exam
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Contents
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| Week 1: |
İntroduction, Rules, Lab books, lab safety and Equipments |
| Week 2: |
Pipetting exercises, Solution preparation
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| Week 3: |
Introduction to cell culture and Medium preperation, |
| Week 4: |
Subculturing of cells (Cell seeding, counting and preparation of cell stocks) |
| Week 5: |
Cell proliferation assay and cell lysis |
| Week 6: |
Western/Immunoblot
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| Week 7: |
Differentiation experiments
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| Week 8: |
Plasmid isolation, Cell transfection
Reporter gen analysisi
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| Week 9: |
RNA isolation
cDNA synthesis,
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| Week 10: |
PCR |
| Week 11: |
Agorose gel electrophoresis |
| Week 12: |
Apoptosis and experiments |
| Week 13: |
Cell cycle/Flow cytometry |
| Week 14: |
CrisperCas9 |
| Week 15*: |
General discussion |
| Week 16*: |
Evaluation of lab books |
| Textbooks and materials: |
Lab book |
| Recommended readings: |
Current Protocols in Molecular Biology by Frederick M. Ausubel (1995)
Short Protocols in Molecular Biology (2 volume set) by Frederick M. Ausubel, Roger Brent, Robert E. Kingston and David D. Moore (2002),
Molecular Cloning: A Laboratory Manual, Third Edition (3 volume set) by Joseph Sambrook, David W. Russell, Tom Maniatis and E. F. Fritsch (2001)
Basic Cell Culture Protocols (Methods in Molecular Biology) by Cheryl D. Helgason and Cindy Miller (2004) and Recent papers. |
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* Between 15th and 16th weeks is there a free week for students to prepare for final exam.
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Assessment
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| Method of assessment |
Week number |
Weight (%) |
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| Mid-terms: |
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0 |
| Other in-term studies: |
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40 |
| Project: |
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0 |
| Homework: |
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0 |
| Quiz: |
2,3,4,5,6,7,8,9,12,13 |
60 |
| Final exam: |
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0 |
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Total weight: |
(%) |
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Workload
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| Activity |
Duration (Hours per week) |
Total number of weeks |
Total hours in term |
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| Courses (Face-to-face teaching): |
3 |
14 |
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| Own studies outside class: |
3 |
12 |
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| Practice, Recitation: |
3 |
12 |
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| Homework: |
0 |
0 |
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| Term project: |
0 |
0 |
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| Term project presentation: |
0 |
0 |
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| Quiz: |
1 |
10 |
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| Own study for mid-term exam: |
0 |
0 |
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| Mid-term: |
0 |
0 |
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| Personal studies for final exam: |
0 |
0 |
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| Final exam: |
0 |
0 |
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Total workload: |
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Total ECTS credits: |
* |
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* ECTS credit is calculated by dividing total workload by 25.
(1 ECTS = 25 work hours)
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