• Basic information
• Learning outcomes
• Contents
• Assessment
• Workload

Learning outcomes

Upon successful completion of this course, students will be able to:

1. Recognise the principles of molecular biological techniques

   Contribution to Program Outcomes

   1. To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
   2. To be able to comprehend the history and nature of scientific thinking and to apply them to problems in the field.
   3. To be able to follow current scientific and technological innovations with the awareness of continuous learning and to apply them in the field.
   4. To be able to drive hypotheses using existing knowledge, designing and conducting experiment for problem solving and make correct interpretation of the results obtained from the experiment.
   5. To be able to apply biological concepts to individual, social, economic, technologic and environmental issues and to develop sustainable approaches for problem solving.

   Method of assessment

   1. Written exam
2. Apply knowledge about recombinant DNA techniques to the laboratory experiments

   Contribution to Program Outcomes

   1. To be able to work individually, make independent decisions and participate actively in multidisciplinary group studies.
   2. To be able to follow current scientific and technological innovations with the awareness of continuous learning and to apply them in the field.
   3. To be able to drive hypotheses using existing knowledge, designing and conducting experiment for problem solving and make correct interpretation of the results obtained from the experiment.
   4. To be able to apply biological concepts to individual, social, economic, technologic and environmental issues and to develop sustainable approaches for problem solving.

   Method of assessment

   1. Written exam
   2. Homework assignment
   3. Seminar/presentation
3. Build up knowledge about various genome projects and developments about molecular biotechnology

   Contribution to Program Outcomes

   1. To be able to follow current scientific and technological innovations with the awareness of continuous learning and to apply them in the field.
   2. To be able to drive hypotheses using existing knowledge, designing and conducting experiment for problem solving and make correct interpretation of the results obtained from the experiment.
   3. To be able to present their studies in scientific talks and written texts in national and international scientific platforms by a foreign language.
   4. To be able to apply biological concepts to individual, social, economic, technologic and environmental issues and to develop sustainable approaches for problem solving.

   Method of assessment

   1. Seminar/presentation

Contents
Week 1:	Introduction to molecular biotechnology: Decsription, history, application areas and general information about the course
Week 2:	Isolation and electrophoresis of nucleic acids
Week 3:	Use of enzymes in the modification of nucleic acids: Restriction endonucleases and modification enzymes (nucleases, ligases, polymerases, phosphatases, polynucleotide kinases)
Week 4:	Polymerase chain reaction and its applications
Week 5:	Vectors and their use in biotechnology
Week 6:	Mid-term Exam I Evaluation of the exam and solving the exam questions
Week 7:	Molecular cloning strategies: Genomic DNA and cDNA libraries, PCR cloning, screening and confirmation methods
Week 8:	Sequencing genes and genomes: Chain-termination DNA sequencing, next-generation sequencing, shotgun sequencing approaches
Week 9:	Studying gene expression and function
Week 10:	Studying genomes
Week 11:	Mid-term Exam II Evaluation of the exam and solving the exam questions
Week 12:	Studying transcriptomes and proteomes Student Presentations
Week 13:	Recombinant protein production
Week 14:	Applications of molecular biotechnology
Week 15*:	-
Week 16*:	Final Exam
Textbooks and materials:
Recommended readings:	1. Brown T. A., (2021), "Gene Cloning and DNA Analysis: An Introduction", 8th Ed., Blackwell Publishing. 2. Wink, M., (2006), "An Introduction to Molecular Biotechnology: Molecular Fundamentals, Methods and Applications in Modern Biotechnology", Wiley-VCH. 3. Primrose S. B., Twyman R. M., (2006), "Principles of Gene Manuplation and Genomics", 7th Ed., Blackwell Publishing.
	* Between 15th and 16th weeks is there a free week for students to prepare for final exam.

Assessment
Method of assessment	Week number	Weight (%)
Mid-terms:	6, 11	40
Other in-term studies:		0
Project:	12	10
Homework:		0
Quiz:		0
Final exam:	16	50
	Total weight:	(%)

Workload
Activity	Duration (Hours per week)	Total number of weeks	Total hours in term
Courses (Face-to-face teaching):	3	14
Own studies outside class:	3	12
Practice, Recitation:	0	0
Homework:	0	0
Term project:	2	3
Term project presentation:	0.5	1
Quiz:	0	0
Own study for mid-term exam:	10	2
Mid-term:	2	2
Personal studies for final exam:	10	2
Final exam:	2	1
		Total workload:
		Total ECTS credits:	*
	* ECTS credit is calculated by dividing total workload by 25. (1 ECTS = 25 work hours)