Syllabus ( MBG 426 )
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Basic information
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| Course title: |
Protein Targeting |
| Course code: |
MBG 426 |
| Lecturer: |
Assoc. Prof. Dr. F. İnci ÖZDEMİR
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| ECTS credits: |
5 |
| GTU credits: |
3 (3+0+0) |
| Year, Semester: |
4, Spring |
| Level of course: |
First Cycle (Undergraduate) |
| Type of course: |
Area Elective
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| Language of instruction: |
English
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| Mode of delivery: |
Face to face
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| Pre- and co-requisites: |
None |
| Professional practice: |
No |
| Purpose of the course: |
To teach various selected aspects of protein structure and function including protein synthesis, folding, targeting and trafficking and protein degradation within the cell. |
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Learning outcomes
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Upon successful completion of this course, students will be able to:
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Explain protein translation in eukaryotic and prokaryotic cells.
Contribution to Program Outcomes
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To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
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To be able to understand the relationship between matter and energy in organisms.
Method of assessment
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Written exam
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Understand the mechanisms which target proteins to a number of compartments, the major posttranslational modifications occurring throughout the secretory pathway, and the basis of the molecular specificity of vesicular transport.
Contribution to Program Outcomes
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To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
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To be able to define the structure-function relationship at the molecular level in cells and organisms.
Method of assessment
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Written exam
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Build up knowledge about the different protein degradation mechanisms.
Contribution to Program Outcomes
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To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
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To be able to understand the relationship between matter and energy in organisms.
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To be able to define the structure-function relationship at the molecular level in cells and organisms.
Method of assessment
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Written exam
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Gain knowledge of how specific eukaryotic proteins are targeted to the plasma membrane, extracellular matrix or cell wall, ER, vacuole, golgi, chloroplast, mitochondria, nucleus and cytoplasm.
Contribution to Program Outcomes
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To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
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To be able to understand the relationship between matter and energy in organisms.
Method of assessment
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Written exam
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Contents
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| Week 1: |
Protein Synthesis in Prokaryotes |
| Week 2: |
Protein Synthesis in Eukaryotes. |
| Week 3: |
Protein Folding: Chaperon Systems |
| Week 4: |
Synthesis and Localization of Lyzosomal and Membrane Proteins |
| Week 5: |
Protein Modifications and Quality Control in Endoplasmic Reticulum. |
| Week 6: |
Vesicular Transport Mechanism. |
| Week 7: |
Midterm Exam |
| Week 8: |
Protein Transport in Bacteria |
| Week 9: |
Targeting of Mitochondrial and Chloroplast Proteins.
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| Week 10: |
Nuclear İmport and Export of Proteins. |
| Week 11: |
Targeting of Peroxisomal Proteins |
| Week 12: |
Ubiquitin-Mediated Degradation: Ubiquitination, Degradation of Ubiquitinated Proteins, Proteasomes. |
| Week 13: |
Protein Degradation: Proteases |
| Week 14: |
Term Paper Presentation |
| Week 15*: |
General review. |
| Week 16*: |
Final exam |
| Textbooks and materials: |
Protein targeting, transport & translocation edited by Ross E. Dalbey, Gunnar von Heijne. |
| Recommended readings: |
1.Protein biotechnology / Gary Walsh, Denis R. Headon 2.Structure and mechanism in protein science : a guide to enzyme catalysis and protein folding ,1999 / Alan Fersht. 3.Guidebook to the molecular chaperones and protein-folding catalysts,1997 / edited by Mary-Jane Gething 4.Molecular Cell Biology ,1995/ Lodish, et al. 5.Introduction to protein structure,1999 / Carl Branden, John Tooze |
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* Between 15th and 16th weeks is there a free week for students to prepare for final exam.
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Assessment
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| Method of assessment |
Week number |
Weight (%) |
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| Mid-terms: |
7 |
25 |
| Other in-term studies: |
12 |
25 |
| Project: |
0 |
0 |
| Homework: |
14 |
10 |
| Quiz: |
0 |
0 |
| Final exam: |
16 |
40 |
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Total weight: |
(%) |
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Workload
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| Activity |
Duration (Hours per week) |
Total number of weeks |
Total hours in term |
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| Courses (Face-to-face teaching): |
3 |
14 |
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| Own studies outside class: |
1 |
14 |
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| Practice, Recitation: |
0 |
0 |
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| Homework: |
1 |
1 |
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| Term project: |
1 |
1 |
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| Term project presentation: |
1 |
1 |
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| Quiz: |
0 |
0 |
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| Own study for mid-term exam: |
6 |
2 |
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| Mid-term: |
2 |
1 |
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| Personal studies for final exam: |
4 |
14 |
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| Final exam: |
2 |
1 |
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Total workload: |
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Total ECTS credits: |
* |
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* ECTS credit is calculated by dividing total workload by 25.
(1 ECTS = 25 work hours)
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