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Syllabus ( MBG 441 )


   Basic information
Course title: Plant Biotechnology
Course code: MBG 441
Lecturer: Prof. Dr. Yelda ÖZDEN ÇİFTÇİ
ECTS credits: 5
GTU credits: 3 (3+0+0)
Year, Semester: 4, Fall
Level of course: First Cycle (Undergraduate)
Type of course: Area Elective
Language of instruction: English
Mode of delivery: Face to face
Pre- and co-requisites: None
Professional practice: No
Purpose of the course: Give fundamental concept of plant biotechnology and to inform the potentials of plant biotechnology
   Learning outcomes Up

Upon successful completion of this course, students will be able to:

  1. Use the techniques applied in conservation of plant biodiversity.

    Contribution to Program Outcomes

    1. To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
    2. To be able to apply biological concepts to individual, social, economic, technologic and environmental issues and to develop sustainable approaches for problem solving.

    Method of assessment

    1. Homework assignment
    2. Laboratory exercise/exam
  2. Produce solutions for the problems faced in conservation of plant biodiversity.

    Contribution to Program Outcomes

    1. To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
    2. To be able to apply biological concepts to individual, social, economic, technologic and environmental issues and to develop sustainable approaches for problem solving.

    Method of assessment

    1. Written exam
  3. Apply molecular techniques used in the classification of biodiversity.

    Contribution to Program Outcomes

    1. To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
    2. To be able to drive hypotheses using existing knowledge, designing and conducting experiment for problem solving and make correct interpretation of the results obtained from the experiment.

    Method of assessment

    1. Homework assignment
    2. Laboratory exercise/exam
   Contents Up
Week 1: The Role of Plant Biotechnology in the World and Its Development
Week 2: Usage of DNA Markers in Plant Biotechnology
-RFLP, PCR, RAPD, AP-PCR, DAF, AFLP, STMS, ISSR
Week 3: Plant Tissue Culture
-Plant growth regulators
-Methods (meristem culture and organogenesis)
-Methods (somatic embryogenesis, cell suspansion, anther and protoplast cultures)
Week 4: Applications of Plant Tissue Culture
-Micropropagation
-Production of synthetic seeds
-Haploid plant production
-Somatic hybridization

Week 5: In vitro Plant Stress and somaclonal variations
-The role of phytohormones
-The role of etylene
-The methods used for the identification of somaclonal variations
Week 6: Mid-term Exam / Transgenic Plant Production and Methodology
-Description and History
-Gene transfer via Agrobacterium
-Direct gene transfer methods

Week 7: Biotechnogical engineering plants to gain resistance to biotic and abiotic stress
Week 8: Production of Secondary Metabolites
-Terpenes
-Alkaloids
-Phenly propanoids
-Flavonoids
-Production in cell suspansion cultures and organ cultures
Week 9: Protein targeting and optimization of protein products through plant biotech.

-Phenly propanoids
-Flavonoids
-Production in cell suspansion cultures and organ cultures
Week 10: Production of vaccines, antibodies, bioplastic and other novel biomaterials

Week 11: Legal regulations and ethics of transgenic plant production
Week 12: Plant Omics
-Genomics
-Transcriptomics
-Proteomics
-Metabolomics
-Lipidomics
Week 13: The Usage of Bioreactors for the Mass Propagation of Plants
-Automation and liquid cultures
-Temporary immersion bioreactors
The Usage of Bioreactors for the Mass Propagation of Plants
-Automation and liquid cultures
-Temporary immersion bioreactors



Week 14: Medium and Long Term Conservation Techniques Used for Preservation of Plant Germplasms
-In vitro conservation techniques
-Cryopreservation (dehydration ve vitrification techniques)
-Cryopreservation (encapsulation/dehydration and encapsulation/vitrification methods)
Week 15*: -
Week 16*: Final Exam
Textbooks and materials: Plant Biotechnology: Experience and Future Prospects. Agnes Ricroch et al (Editors)springer. 2nd edition. 2021.
Recommended readings: 1.Plant Biotechnology: Progress in Genomic Era. Springer. 2020.
2.From Plant Genomics to Plant Biotechnology (Woodhead Publishing Series in Biomedicine).2013
3. Plant Biotechnology: Genetics, Genomics and Breeding. Syrawood Publishing House. 2017
4. Plant Biotechnology: Experience and Future Prospects. Springer.2016.
  * Between 15th and 16th weeks is there a free week for students to prepare for final exam.
Assessment Up
Method of assessment Week number Weight (%)
Mid-terms: 6 30
Other in-term studies: 0 0
Project: 0 0
Homework: 0 0
Quiz: 1-15 10
Final exam: 16 60
  Total weight:
(%)
   Workload Up
Activity Duration (Hours per week) Total number of weeks Total hours in term
Courses (Face-to-face teaching): 3 14
Own studies outside class: 2 14
Practice, Recitation: 0 0
Homework: 0 0
Term project: 0 0
Term project presentation: 0 0
Quiz: 3 3
Own study for mid-term exam: 6 3
Mid-term: 3 1
Personal studies for final exam: 6 4
Final exam: 3 1
    Total workload:
    Total ECTS credits:
*
  * ECTS credit is calculated by dividing total workload by 25.
(1 ECTS = 25 work hours)
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