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Syllabus ( MBG 443 )


   Basic information
Course title: Plant Metabolic Engineering
Course code: MBG 443
Lecturer: Assoc. Prof. Dr. Fatma AYDINOĞLU
ECTS credits: 5
GTU credits: 3 (3+0+0)
Year, Semester: 4, Fall and Spring
Level of course: First Cycle (Undergraduate)
Type of course: Area Elective
Language of instruction: English
Mode of delivery: Face to face
Pre- and co-requisites: no
Professional practice: No
Purpose of the course: Grasping types, biosynthesis and regulation of plant secondary metabolites. Becoming familiar with the genetic engineering techniques to improve the production of plant secondary metabolite by functional genomics and omics technologies approach.
   Learning outcomes Up

Upon successful completion of this course, students will be able to:

  1. List the plant natural secondary metabolites and explain biosynthesis and regulation of each one.

    Contribution to Program Outcomes

    1. To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
    2. To be able to understand the relationship between matter and energy in organisms.

    Method of assessment

    1. Written exam
    2. Homework assignment
  2. Identify the appropriate techniques and strategies for isolation and characterization of a secondary metabolite from a plant.

    Contribution to Program Outcomes

    1. To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
    2. To be able to understand the relationship between matter and energy in organisms.
    3. To be able to drive hypotheses using existing knowledge, designing and conducting experiment for problem solving and make correct interpretation of the results obtained from the experiment.
    4. To be able to apply biological concepts to individual, social, economic, technologic and environmental issues and to develop sustainable approaches for problem solving.

    Method of assessment

    1. Written exam
    2. Homework assignment
  3. List the practical usage area of plant secondary metabolites in biotechnology and explain each one.

    Contribution to Program Outcomes

    1. To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
    2. To be able to apply biological concepts to individual, social, economic, technologic and environmental issues and to develop sustainable approaches for problem solving.

    Method of assessment

    1. Written exam
    2. Homework assignment
   Contents Up
Week 1: Introduction to Plant metabolic engineering Course, Objectives of Plant Metabolic Engineering
Week 2: Functional genetics approaches in Plant Metobolic Engineering: Forward genetics and reverse genetics, Phytodetoxification of TNT, Engineer ß-carotene synthesis in rice and tomato, Engineer flavonoid production in tomato, Producing decaffeinated coffee plants, Engineer indole alkoloid bipsynthesis in Catharanthus
Week 3: Metabolism and Metabolic pathways, Basic chemical concepts
Week 4: Plant metabolism, Carbon metabolism in plants
Week 5: Plant primary metabolites: Carbohydrates, Fatty acids, Amino acids, Nucleotids, Phytosterols, Phytohormones
Week 6: Plant secondary metabolites and Classification according to Phsiological effects, Chemical structure and Their occurence, The role of plant secondary metabolites in plant defense,
Week 7: Midterm exam - Examples of metabolic engineering in plants: Manipulate lysine content in seeds, Production of waxes in oilseed plants, Manipulate strach levels in potato
Week 8: Examples of metabolic engineering in plants: Induction of male sterility Enhance salt tolerance, Production of bioplastics, Trophic conversion of photoautotrophic organism
Week 9: Terponoids: Metabolic engineering of Terponoids' biosynthesis
Week 10: Alkoloids: Metabolic engineering of Alkoloids' biosynthesis
Week 11: Phenylpropanoids/phenolic compounds: Metabolic engineering of Phenylpropanoid and phenylpropanoid-acetate pathway metabolites
Week 12: Biosynthesis of lignans, lignins, and suberization, Metabolic engineering of Flavonoid biosynthesis
Week 13: Coumarins, stilbens, strylpyrones and arylpyrones, Fibers, pigments, pharmaceuticals and flavoring agents production
Week 14: Omics Technologies in Plant Metobolic Engineering, Metabolomics, Metabolic profiling methods
Week 15*: Make-up
Week 16*: Final exam
Textbooks and materials: 1- Metabolic Engineering of Plant Secondary Metabolism, R. Verpoorte, A. Wilhelm Alfermann, Springer, 2000.
2- Biochemistry & Molecular Biology of Plants, Bob Buchanan, Wilhelm Gruissem, Russell Jones (Ed.), 2002.
3- Plant Biochemistry, Fourth Edition, Hans-Walter Heldt, Birgit Piechulla, 2010.
Recommended readings: 1- Plant Secondary Metabolism Engineering, Methods and Applications Vol. 643, Arthur Germano Fett-Neto (Ed), Humana Press, 2010.
2- Plant Physiology, Fifth Edition, Lincoln Taiz, 2010.
  * Between 15th and 16th weeks is there a free week for students to prepare for final exam.
Assessment Up
Method of assessment Week number Weight (%)
Mid-terms: 7 30
Other in-term studies: 0
Project: 0
Homework: 11,12,13,14 30
Quiz: 0
Final exam: 16 40
  Total weight:
(%)
   Workload Up
Activity Duration (Hours per week) Total number of weeks Total hours in term
Courses (Face-to-face teaching): 3 14
Own studies outside class: 2 14
Practice, Recitation: 0 0
Homework: 6 2
Term project: 0 0
Term project presentation: 0 0
Quiz: 0 0
Own study for mid-term exam: 8 2
Mid-term: 3 1
Personal studies for final exam: 10 2
Final exam: 3 1
    Total workload:
    Total ECTS credits:
*
  * ECTS credit is calculated by dividing total workload by 25.
(1 ECTS = 25 work hours)
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