ECTS @ IUE ECTS @ IUE ECTS @ IUE ECTS @ IUE ECTS @ IUE ECTS @ IUE ECTS @ IUE

Syllabus ( BENG 302 )


   Basic information
Course title: Bioengineering Laboratory II
Course code: BENG 302
Lecturer: Assoc. Prof. Dr. Bengü ERGÜDEN
ECTS credits: 4
GTU credits: 2 ()
Year, Semester: 3, Spring
Level of course: First Cycle (Undergraduate)
Type of course: Compulsory
Language of instruction: English
Mode of delivery:  , Lab work
Pre- and co-requisites: None
Professional practice: No
Purpose of the course: The aim of the course is to develop the application practices of the students, to learn and apply advanced techniques, to design and carry out the experiments themselves.
With this course, it is aimed to prepare students for business life and to enable them to design, develop and apply studies in accordance with bioengineering disciplines, and to recognize and learn many devices to be used in laboratory applications.
   Learning outcomes Up

Upon successful completion of this course, students will be able to:

  1. Use device of bioreactor and measurement techniques

    Contribution to Program Outcomes

    1. Acquire knowledge on biological, chemical, physical and mathematical principles which constitute the basis of bioengineering applications
    2. Acquire knowledge on current bioengineering applications from the industrial and scientific aspects
    3. Acquire knowledge for research methods which are required to develop novel application methods
    4. Convert biological, chemical, physical and mathematical principles into novel applications for the benefit of society,
    5. Find out new methods to improve his/her knowledge.

    Method of assessment

    1. Homework assignment
    2. Laboratory exercise/exam
  2. Apply advanced laboratory techniques

    Contribution to Program Outcomes

    1. Acquire knowledge for research methods which are required to develop novel application methods
    2. Convert biological, chemical, physical and mathematical principles into novel applications for the benefit of society,
    3. Conduct and develop bioengineering applications for relevant sectors such as health and agricultural industry.
    4. Design processes for the investigation of bioengineering problems, collect data, analyze and interpret the results.
    5. Understand the social and global importance of proposed solutions

    Method of assessment

    1. Homework assignment
    2. Laboratory exercise/exam
  3. Define the management of processes in experimental studies

    Contribution to Program Outcomes

    1. Acquire knowledge on biological, chemical, physical and mathematical principles which constitute the basis of bioengineering applications
    2. Design processes for the investigation of bioengineering problems, collect data, analyze and interpret the results.
    3. Work effectively in multi-disciplinary research teams
    4. Find out new methods to improve his/her knowledge.
    5. Demonstrate sufficiency in English to follow literature, present technical projects and write articles

    Method of assessment

    1. Homework assignment
    2. Laboratory exercise/exam
   Contents Up
Week 1: Laboratory information and separation of groups
Week 2: Biosafety and Health
Week 3: Experiment 1. Medium and Cell Culture Preparation
Quiz
Homework
Week 4: Experiment 2. Cell disruption by physical and enzymatic methods
Quiz
Homework
Week 5: Experiment 3. Column Chromatography (Enzyme purification by His-Trap Column)
Quiz
Homework
Week 6: Experiment 4. SDS Polyacrylamide Gel Electrophoresis of Proteins I : Preparation of gels and buffers
Quiz
Homework
Week 7: Experiment 5. SDS Polyacrylamide Gel Electrophoresis of Proteins II : Denaturation of proteins and running the SDS-PAGE gels
Quiz
Homework
Week 8: Experiment 6. Determination of kinetic properties of certain enzyme
Quiz
Homework
Week 9: Experiment 7. Enzyme Modeling (Autodock)
Quiz
Homework
Week 10: Experiment 8. Biodiesel production from waste oils
Quiz
Homework
Week 11: Experiment 9. Operation of bioreactor
Quiz
Homework
Week 12: Experiment 10. Kinetic-Parameter Dependent Modeling
Quiz
Homework
Week 13: Experiment 11. High Pressure Liquid Chromatography (HPLC)
Quiz
Homework
Week 14: Combination of Experiment Flow
Week 15*: -
Week 16*: Final Exam
Textbooks and materials: Laboratuvar Föyü / Laboratory Manual
Recommended readings: Garcia, A., & Durand, C. (2009). Bioengineering: Principles, methodologies and applications (Hauppauge, NY: Nova Science Publisher's).
  * Between 15th and 16th weeks is there a free week for students to prepare for final exam.
Assessment Up
Method of assessment Week number Weight (%)
Mid-terms: 0 0
Other in-term studies: 0 0
Project: 0 0
Homework: 3,4,5,6,7,8,9,10,11,12,13 60
Quiz: 3,4,5,6,7,8,9,10,11,12,13 5
Final exam: 16 35
  Total weight:
(%)
   Workload Up
Activity Duration (Hours per week) Total number of weeks Total hours in term
Courses (Face-to-face teaching): 0 0
Own studies outside class: 0 0
Practice, Recitation: 4 14
Homework: 3 11
Term project: 0 0
Term project presentation: 0 0
Quiz: 0.5 11
Own study for mid-term exam: 0 0
Mid-term: 0 0
Personal studies for final exam: 2 11
Final exam: 3 1
    Total workload:
    Total ECTS credits:
*
  * ECTS credit is calculated by dividing total workload by 25.
(1 ECTS = 25 work hours)
-->