• Basic information
• Learning outcomes
• Contents
• Assessment
• Workload

Learning outcomes

Upon successful completion of this course, students will be able to:

1. Apply plant tissue culture methods on different plants.

   Contribution to Program Outcomes

   1. Acquire knowledge on biological, chemical, physical and mathematical principles which constitute the basis of bioengineering applications
   2. Acquire knowledge on current bioengineering applications from the industrial and scientific aspects
   3. Acquire knowledge for research methods which are required to develop novel application methods
   4. Convert biological, chemical, physical and mathematical principles into novel applications for the benefit of society,
   5. Conduct and develop bioengineering applications for relevant sectors such as health and agricultural industry.
   6. Demonstrate sufficiency in English to follow literature, present technical projects and write articles

   Method of assessment

   1. Written exam
   2. Seminar/presentation
2. Perform in vitro production of phytochemicals for medical, cosmetic and industrial applications.

   Contribution to Program Outcomes

   1. Acquire knowledge on biological, chemical, physical and mathematical principles which constitute the basis of bioengineering applications
   2. Acquire knowledge on current bioengineering applications from the industrial and scientific aspects
   3. Convert biological, chemical, physical and mathematical principles into novel applications for the benefit of society,
   4. Conduct and develop bioengineering applications for relevant sectors such as health and agricultural industry.
   5. Demonstrate sufficiency in English to follow literature, present technical projects and write articles

   Method of assessment

   1. Written exam
   2. Seminar/presentation
3. Use plant tissue culture applications for molecular biology and genetic engineering studies.

   Contribution to Program Outcomes

   1. Acquire knowledge on biological, chemical, physical and mathematical principles which constitute the basis of bioengineering applications
   2. Acquire knowledge on current bioengineering applications from the industrial and scientific aspects
   3. Acquire knowledge for research methods which are required to develop novel application methods
   4. Convert biological, chemical, physical and mathematical principles into novel applications for the benefit of society,
   5. Conduct and develop bioengineering applications for relevant sectors such as health and agricultural industry.
   6. Demonstrate sufficiency in English to follow literature, present technical projects and write articles

   Method of assessment

   1. Written exam
   2. Seminar/presentation
4. Establish a plant tissue culture laboratory.

   Contribution to Program Outcomes

   1. Acquire knowledge on biological, chemical, physical and mathematical principles which constitute the basis of bioengineering applications
   2. Acquire knowledge on current bioengineering applications from the industrial and scientific aspects
   3. Acquire knowledge for research methods which are required to develop novel application methods
   4. Convert biological, chemical, physical and mathematical principles into novel applications for the benefit of society,
   5. Conduct and develop bioengineering applications for relevant sectors such as health and agricultural industry.
   6. Work effectively in multi-disciplinary research teams
   7. Demonstrate sufficiency in English to follow literature, present technical projects and write articles

   Method of assessment

   1. Written exam
   2. Seminar/presentation

Contents
Week 1:	Introduction to Plant Tissue Culture and History
Week 2:	Botanical Basis for Tissue Culture
Week 3:	Setup of a Tissue Culture Laboratory
Week 4:	Plant Tissue Culture Media and Their Components
Week 5:	Explant Preparation and Biological Contaminants
Week 6:	Callus and Cell Suspension Culture - Paper Discussion
Week 7:	Organogenesis - Paper Discussion
Week 8:	Somatic Embryogenesis - Midterm Exam
Week 9:	Meristem Culture and Virus-Free Plants - Paper Discussion
Week 10:	Haploid Culture - Paper Discussion
Week 11:	Embryo Culture and Embryo Rescue - Paper Discussion
Week 12:	Protoplast Culture and Somatic Hybridization - Paper Discussion
Week 13:	Hardening off
Week 14:	TC products - Student Presentations
Week 15*:	-
Week 16*:	Final Exam
Textbooks and materials:	Plant Tissue Culture: Techniques and Experiments, 3rd Edition. Smith, Roberta H. Published by Academic Press (2013). ISBN 10: 9382291768
Recommended readings:	Plant Tissue Culture, Development, and Biotechnology, R.N. Trigiano, D.J. Gray, 2011, CRC Press (2010).Published by ISBN-10 : 1420083260 Plants From Test Tubes An Introduction to Micro-Propagation, 4th Edition. Lydiane Kyte, John Kleyn, Holly Scoggins, Mark Bridgen Published by Timber Press (2013) ISBN-10 : 1604692065
	* Between 15th and 16th weeks is there a free week for students to prepare for final exam.

Assessment
Method of assessment	Week number	Weight (%)
Mid-terms:	8	30
Other in-term studies:	6, 7, 9, 10, 11, 12	20
Project:	14	20
Homework:		0
Quiz:		0
Final exam:	16	30
	Total weight:	(%)

Workload
Activity	Duration (Hours per week)	Total number of weeks	Total hours in term
Courses (Face-to-face teaching):	3	14
Own studies outside class:	3	14
Practice, Recitation:	0	0
Homework:	2	6
Term project:	10	1
Term project presentation:	1	1
Quiz:	0	0
Own study for mid-term exam:	10	1
Mid-term:	3	1
Personal studies for final exam:	6	1
Final exam:	3	1
		Total workload:
		Total ECTS credits:	*
	* ECTS credit is calculated by dividing total workload by 25. (1 ECTS = 25 work hours)