• Basic information
• Learning outcomes
• Contents
• Assessment
• Workload

Learning outcomes

Upon successful completion of this course, students will be able to:

1. define yeast life cycle and its importance as a model organism

   Contribution to Program Outcomes

   1. Acquire knowledge on biological, chemical, physical and mathematical principles which constitute the basis of bioengineering applications
   2. Convert biological, chemical, physical and mathematical principles into novel applications for the benefit of society,

   Method of assessment

   1. Written exam
2. design genetic manipulations to obtain different yeast strains.

   Contribution to Program Outcomes

   1. Acquire knowledge on biological, chemical, physical and mathematical principles which constitute the basis of bioengineering applications
   2. Acquire knowledge on current bioengineering applications from the industrial and scientific aspects
   3. Convert biological, chemical, physical and mathematical principles into novel applications for the benefit of society,

   Method of assessment

   1. Written exam
3. design genomic analyses using various yeast strains.

   Contribution to Program Outcomes

   1. Acquire knowledge on biological, chemical, physical and mathematical principles which constitute the basis of bioengineering applications
   2. Acquire knowledge on current bioengineering applications from the industrial and scientific aspects
   3. Convert biological, chemical, physical and mathematical principles into novel applications for the benefit of society,

   Method of assessment

   1. Written exam

Contents
Week 1:	Yeast as a model eukaryote, its growth and life cycles
Week 2:	The yeast genome, genetic nomenclature
Week 3:	Genetic analyses: Tetrad analysis, complementation, suppression
Week 4:	Transformation: Yeast vectors and DNA fragments
Week 5:	Quiz; Yeast vectors: Yip, Yep, Ycp vectors
Week 6:	Genes important for genetic studies: URA3, LYS2, LEU2, TRP1, ADE1 and ADE2, GAL1 promoter, LacZ and other reporters
Week 7:	Quiz; Cloning by complementation, mutagenesis, gene disruption and one-step gene replacement
Week 8:	Midterm Exam, dicussion of the exam questions
Week 9:	Genomic analysis, recovering mutant alleles
Week 10:	Two-hybrid systems, yeast artificial chromosomes (yacs)
Week 11:	Yeast in traditional food fermentations, yeast metabolites
Week 12:	Quiz; Production of fine chemicals, biopharmaceuticals
Week 13:	Metabolic engineering in Saccharomyces cerevisiae
Week 14:	Quiz; Agricultural, biocontrol and environmental applications
Week 15*:	-
Week 16*:	Final Exam
Textbooks and materials:	Molecular Biology of the Cell. Bruce Alberts, Dennis Bray, James Watson, and Julian Lewis. 6th Edition, 2015. Garland Science.
Recommended readings:	1) Karp's Cell and Molecular Biology: Concepts and Experiments, Gerald Karp, Janet Iwasa, Wallace Marshall, 8th Edition. Wiley. 2) Molecular Cell Biology. Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger, Matthew P. Scott, Anthony Bretscher, Hidde Ploegh, Paul Matsudaira. 8th Edition. Macmillan Learning.
	* Between 15th and 16th weeks is there a free week for students to prepare for final exam.

Assessment
Method of assessment	Week number	Weight (%)
Mid-terms:	8	30
Other in-term studies:		0
Project:		0
Homework:		0
Quiz:	5, 7, 12, 14	20
Final exam:	16	50
	Total weight:	(%)

Workload
Activity	Duration (Hours per week)	Total number of weeks	Total hours in term
Courses (Face-to-face teaching):	3	13
Own studies outside class:	4	14
Practice, Recitation:	0	0
Homework:	0	0
Term project:	0	0
Term project presentation:	0	0
Quiz:	1	4
Own study for mid-term exam:	10	1
Mid-term:	2	1
Personal studies for final exam:	15	1
Final exam:	2	1
		Total workload:
		Total ECTS credits:	*
	* ECTS credit is calculated by dividing total workload by 25. (1 ECTS = 25 work hours)