Syllabus ( BENG 460 )
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Basic information
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| Course title: |
Advanced Methods in Yeast Genetics |
| Course code: |
BENG 460 |
| Lecturer: |
Assoc. Prof. Dr. Bengü ERGÜDEN
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| ECTS credits: |
5 |
| GTU credits: |
3 () |
| Year, Semester: |
4, Spring |
| Level of course: |
First Cycle (Undergraduate) |
| Type of course: |
Area Elective
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| Language of instruction: |
English
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| Mode of delivery: |
, Lab work
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| Pre- and co-requisites: |
BENG 223, BENG 301 |
| Professional practice: |
No |
| Purpose of the course: |
To introduce protocols and recipes for advanced techniques and reagents used in the study of yeast biology. |
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Learning outcomes
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Upon successful completion of this course, students will be able to:
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follow protocols for advanced techniques in yeast genetics.
Contribution to Program Outcomes
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Acquire knowledge on biological, chemical, physical and mathematical principles which constitute the basis of bioengineering applications
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Acquire knowledge on current bioengineering applications from the industrial and scientific aspects
Method of assessment
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Laboratory exercise/exam
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carry out genome-wide genetic screen using yeast as a model organism
Contribution to Program Outcomes
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Acquire knowledge on biological, chemical, physical and mathematical principles which constitute the basis of bioengineering applications
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Acquire knowledge on current bioengineering applications from the industrial and scientific aspects
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Design processes for the investigation of bioengineering problems, collect data, analyze and interpret the results.
Method of assessment
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Written exam
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Laboratory exercise/exam
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design and make various genetic manipulations to obtain different yeast strains
Contribution to Program Outcomes
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Acquire knowledge on biological, chemical, physical and mathematical principles which constitute the basis of bioengineering applications
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Acquire knowledge on current bioengineering applications from the industrial and scientific aspects
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Design processes for the investigation of bioengineering problems, collect data, analyze and interpret the results.
Method of assessment
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Written exam
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Laboratory exercise/exam
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Contents
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| Week 1: |
Preparation of yeast media and plates |
| Week 2: |
Yeast culture and growth methods |
| Week 3: |
Homework; Transformation of yeast cells with Snyder gene deletion library |
| Week 4: |
Determination of transformation efficiency |
| Week 5: |
Testing deletion library transformants for hexanoic acid resistance |
| Week 6: |
Homework; Isolation of genomic DNA from hexanoic acid resistant strains |
| Week 7: |
Rescue of mTn3-lacZ/LEU2 plasmid; PCR amplification and preparation of DNA for sequencing |
| Week 8: |
Midterm Exam, dicussion of the exam questions |
| Week 9: |
Evaluation of sequencing results; BLAST to determine library hits |
| Week 10: |
Homework; Cassette PCR for deletion of GENEX, one of the library hits |
| Week 11: |
Transformation of yeast cells with geneXD cassette PCR product |
| Week 12: |
Homework; Testing transformants for deletion with colony PCR |
| Week 13: |
Testing geneXD strain for hexanoic acid resistance |
| Week 14: |
Testing geneXD strain for bioethanol production efficiency |
| Week 15*: |
- |
| Week 16*: |
Final Exam |
| Textbooks and materials: |
Laboratory protocols |
| Recommended readings: |
Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual, David C. Amberg, Daniel J. Burke, Dan Burke, Jeffrey N. Strathern, 2005 Edition. Cold Spring Harbor Laboratory Press. |
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* Between 15th and 16th weeks is there a free week for students to prepare for final exam.
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Assessment
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| Method of assessment |
Week number |
Weight (%) |
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| Mid-terms: |
8 |
30 |
| Other in-term studies: |
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0 |
| Project: |
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0 |
| Homework: |
3, 6, 10, 12 |
30 |
| Quiz: |
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0 |
| Final exam: |
16 |
40 |
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Total weight: |
(%) |
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Workload
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| Activity |
Duration (Hours per week) |
Total number of weeks |
Total hours in term |
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| Courses (Face-to-face teaching): |
3 |
13 |
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| Own studies outside class: |
3 |
14 |
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| Practice, Recitation: |
0 |
0 |
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| Homework: |
5 |
4 |
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| Term project: |
0 |
0 |
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| Term project presentation: |
0 |
0 |
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| Quiz: |
0 |
0 |
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| Own study for mid-term exam: |
10 |
1 |
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| Mid-term: |
2 |
1 |
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| Personal studies for final exam: |
15 |
1 |
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| Final exam: |
2 |
1 |
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Total workload: |
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Total ECTS credits: |
* |
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* ECTS credit is calculated by dividing total workload by 25.
(1 ECTS = 25 work hours)
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