Syllabus ( BENG 462 )
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Basic information
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| Course title: |
Fundamental Methods for Biofabrication |
| Course code: |
BENG 462 |
| Lecturer: |
Assoc. Prof. Dr. Ali AKPEK
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| ECTS credits: |
5 |
| GTU credits: |
3 () |
| Year, Semester: |
4, Spring |
| Level of course: |
First Cycle (Undergraduate) |
| Type of course: |
Elective
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| Language of instruction: |
English
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| Mode of delivery: |
Face to face , Group study , Lab work
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| Pre- and co-requisites: |
None |
| Professional practice: |
No |
| Purpose of the course: |
To introduce protocols and recipes for fundamental techniques and reagents used in the study of biofabrication. |
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Learning outcomes
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Upon successful completion of this course, students will be able to:
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Prepare biomaterials used in molecular biology applications and for growth of tissue structures;
Contribution to Program Outcomes
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Acquire knowledge on biological, chemical, physical and mathematical principles which constitute the basis of bioengineering applications
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Understand design and production processes in bioengineering applications.
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Design processes for the investigation of bioengineering problems, collect data, analyze and interpret the results.
Method of assessment
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Written exam
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Homework assignment
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Laboratory exercise/exam
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Students learn protocols for several techniques that are used for biofabrication.
Contribution to Program Outcomes
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Acquire knowledge for research methods which are required to develop novel application methods
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Understand design and production processes in bioengineering applications.
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Design processes for the investigation of bioengineering problems, collect data, analyze and interpret the results.
Method of assessment
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Written exam
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Homework assignment
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Laboratory exercise/exam
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Will be able to design and develop basic biomaterials.
Contribution to Program Outcomes
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Acquire knowledge on biological, chemical, physical and mathematical principles which constitute the basis of bioengineering applications
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Acquire knowledge for research methods which are required to develop novel application methods
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Design processes for the investigation of bioengineering problems, collect data, analyze and interpret the results.
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Find out new methods to improve his/her knowledge.
Method of assessment
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Written exam
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Homework assignment
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Laboratory exercise/exam
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Will be able to design fabrication and application of predesigned biomaterials for tissue engineering.
Contribution to Program Outcomes
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Acquire knowledge on current bioengineering applications from the industrial and scientific aspects
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Acquire knowledge for research methods which are required to develop novel application methods
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Conduct and develop bioengineering applications for relevant sectors such as health and agricultural industry.
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Work effectively in multi-disciplinary research teams
Method of assessment
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Written exam
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Homework assignment
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Laboratory exercise/exam
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Contents
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| Week 1: |
Biosafety Protocols |
| Week 2: |
Sterilization Protocols |
| Week 3: |
Introduction to Cell Culture - I (Preperation of buffers & media), Homework 1 |
| Week 4: |
Introduction to Cell Culture - II (Fibroblast Cell Culture) |
| Week 5: |
Introduction to Cell Culture - III (Myoblast Cell Culture) |
| Week 6: |
Introduction to Biomaterial Fabrication - I (GelMA Fabrication Process), Homework 2 |
| Week 7: |
Introduction to Biomaterial Fabrication - II (Collagen Fabrication Process) |
| Week 8: |
Introduction to Biomaterial Fabrication - III ( Alginate Fabrication Process) |
| Week 9: |
Midterm Exam, Design and Development of 3D Structures -I |
| Week 10: |
Design and Development of 3D Structures -II, Homework 3 |
| Week 11: |
Introduction to 3D Bioprinting Using FDM based bioprinters for 3D bioprinting |
| Week 12: |
Confocal Microscopy - I Cell Live/Dead Analysis, Homework 4 |
| Week 13: |
Confocal Microscopy - II Cell Proliferation Analysis |
| Week 14: |
Biomechanical Analysis of 3D Structures, Stress-strain Analysis of 3D bioprinted structures. |
| Week 15*: |
- |
| Week 16*: |
Final Exam |
| Textbooks and materials: |
Basic Cell Culture Protocols: Methods in Molecular Biology Volume 75, Second Edition, Edited by Jeffrey W. Pollard, John M. Walker, 1997, Humana Press Inc, Totowa New Jersey. |
| Recommended readings: |
Laboratory protocols |
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* Between 15th and 16th weeks is there a free week for students to prepare for final exam.
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Assessment
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| Method of assessment |
Week number |
Weight (%) |
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| Mid-terms: |
9 |
30 |
| Other in-term studies: |
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0 |
| Project: |
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0 |
| Homework: |
3, 6, 10, 12 |
20 |
| Quiz: |
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0 |
| Final exam: |
16 |
50 |
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Total weight: |
(%) |
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Workload
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| Activity |
Duration (Hours per week) |
Total number of weeks |
Total hours in term |
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| Courses (Face-to-face teaching): |
3 |
13 |
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| Own studies outside class: |
3 |
14 |
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| Practice, Recitation: |
0 |
0 |
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| Homework: |
5 |
4 |
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| Term project: |
0 |
0 |
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| Term project presentation: |
0 |
0 |
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| Quiz: |
0 |
0 |
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| Own study for mid-term exam: |
10 |
1 |
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| Mid-term: |
2 |
1 |
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| Personal studies for final exam: |
15 |
1 |
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| Final exam: |
2 |
1 |
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Total workload: |
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Total ECTS credits: |
* |
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* ECTS credit is calculated by dividing total workload by 25.
(1 ECTS = 25 work hours)
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