• Basic information
• Learning outcomes
• Contents
• Assessment
• Workload

Learning outcomes

Upon successful completion of this course, students will be able to:

1. Identify the methods used in determination and analysis of recombinant proteins.

   Contribution to Program Outcomes

   1. Acquire knowledge on biological, chemical, physical and mathematical principles which constitute the basis of bioengineering applications
   2. Convert biological, chemical, physical and mathematical principles into novel applications for the benefit of society,
   3. Design processes for the investigation of bioengineering problems, collect data, analyze and interpret the results.
   4. Combine, Interpret, and analyze different subfields of bioengineering
   5. Develop an awareness of continuous learning in relation with modern technology.

   Method of assessment

   1. Written exam
   2. Laboratory exercise/exam
2. Define details of the use of site-directed mutagenesis, random mutagenesis, and transformation systems in protein engineering

   Contribution to Program Outcomes

   1. Acquire knowledge on current bioengineering applications from the industrial and scientific aspects
   2. Work effectively in multi-disciplinary research teams
   3. Combine and effectively integrate knowledge acquired from different disciplines.
   4. Develop an awareness of continuous learning in relation with modern technology.

   Method of assessment

   1. Written exam
   2. Laboratory exercise/exam
3. Apply protein purification and industrial analysis.

   Contribution to Program Outcomes

   1. Acquire knowledge on current bioengineering applications from the industrial and scientific aspects
   2. Conduct and develop bioengineering applications for relevant sectors such as health and agricultural industry.
   3. Combine and effectively integrate knowledge acquired from different disciplines.
   4. Demonstrate sufficiency in English to follow literature, present technical projects and write articles

   Method of assessment

   1. Written exam
   2. Laboratory exercise/exam

Contents
Week 1:	Introduction to Enzyme Engineering
Week 2:	Laboratory Safety and Devices
Week 3:	Primer Design for Mutation
Week 4:	PCR (Polymerase Chain Reaction) Application
Week 5:	Midterm exam-I PCR (Polymerase Chain Reaction) Application
Week 6:	Sequencing Technique
Week 7:	Transformation
Week 8:	Preculture Preparation and medium types
Week 9:	Introduction to Induction
Week 10:	Midterm exam-II Continuity to induction
Week 11:	Buffer preparation and purification techniques
Week 12:	SDS-PAGE Analysis
Week 13:	BCA Assay and Enzyme Kinetic
Week 14:	Combination of experiement flow
Week 15*:	-
Week 16*:	Final Exam
Textbooks and materials:
Recommended readings:	Whitford, David. Proteins: structure and function. John Wiley & Sons, 2013. Martin, R. (Ed.). (1998). Protein synthesis: methods and protocols (Vol. 77). Totowa, NJ: Humana Press. Cleland, J. L., & Craik, C. S. (Eds.). (1996). Protein engineering: principles and practice. Wiley-Liss. Grant, G. A. (Ed.). (2002). Synthetic peptides: a user's guide. Oxford University Press on Demand.
	* Between 15th and 16th weeks is there a free week for students to prepare for final exam.

Assessment
Method of assessment	Week number	Weight (%)
Mid-terms:	5,10	40
Other in-term studies:	0	0
Project:	0	0
Homework:	0	0
Quiz:	0	0
Final exam:	16	60
	Total weight:	(%)

Workload
Activity	Duration (Hours per week)	Total number of weeks	Total hours in term
Courses (Face-to-face teaching):	3	14
Own studies outside class:	3	14
Practice, Recitation:	0	0
Homework:	0	0
Term project:	0	0
Term project presentation:	0	0
Quiz:	0	0
Own study for mid-term exam:	2	7
Mid-term:	3	2
Personal studies for final exam:	2	7
Final exam:	3	1
		Total workload:
		Total ECTS credits:	*
	* ECTS credit is calculated by dividing total workload by 25. (1 ECTS = 25 work hours)