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Syllabus ( BENG 464 )


   Basic information
Course title: Advanced Bioprocess Design
Course code: BENG 464
Lecturer: Prof. Dr. Barış BİNAY
ECTS credits: 5
GTU credits: 3 ()
Year, Semester: 4, Spring
Level of course: First Cycle (Undergraduate)
Type of course: Elective
Language of instruction: English
Mode of delivery:  , Lab work
Pre- and co-requisites: None
Professional practice: No
Purpose of the course: This is an advanced overview of topics in bioprocess design using microorganisms, eukaryotic cells (insect, CHO) and recombinant enzymes engineered for industrial applications.

This course focuses on how microbes, cell lines and enzymes are used to manufacture foods, pharmaceuticals, biopharmaceuticals, consumer products and biomaterials

Advanced protein engineering, gene expression and cellular pathway engineering techniques are presented along with methods to screen for and generate improved enzymes as biocatalysts. Industry examples will be presented on how microbial metabolism is “engineered” to generate strains capable of producing: organic acids, amino acids, alcohols, chemical intermediates, lipids, polyketides, carbohydrate and peptide antibiotics, biopolymers (carbohydrate polymers, polyesters), or to alter pathways to generate novel bio-active metabolites.
   Learning outcomes Up

Upon successful completion of this course, students will be able to:

  1. Define the features of bioreactors.

    Contribution to Program Outcomes

    1. Design processes for the investigation of bioengineering problems, collect data, analyze and interpret the results.

    Method of assessment

    1. Laboratory exercise/exam
  2. Define the requirements of bioreactor parameters.

    Contribution to Program Outcomes

    1. Design processes for the investigation of bioengineering problems, collect data, analyze and interpret the results.

    Method of assessment

    1. Written exam
  3. Apply bioreactor operations, calculations and analysis

    Contribution to Program Outcomes

    1. Acquire knowledge on current bioengineering applications from the industrial and scientific aspects

    Method of assessment

    1. Laboratory exercise/exam
   Contents Up
Week 1: Laboratory Safety and Introduction to Bioprocess Design
Week 2: Experiment 1:Preparation of Pre-culture, Main Culture and Cell Glycerol Stocks and Introduction to Stoichiometry
Homework
Week 3: EXPERIMENT 2: Cell Disruption and Enzyme Purification
Homework
Week 4: EXPERIMENT 3: Enzyme Activity, Protein Determination
Homework
Week 5: EXPERIMENT 4: Preparation of Reactor
Homework
Week 6: EXPERIMENT 5: Batch Operation for Alfa-amylase Production from E. coli
Homework
Week 7: EXPERIMENT 6: Batch Fermentation Analysis
Homework
Week 8: Fermentation Analysis Evaluation

Week 9: EXPERIMENT 7: Fed-batch Operation for Alfa-amylase Production from E. coli
Homework
Week 10: EXPERIMENT 8: Fed-batch Operation Analysis
Homework
Week 11: EXPERIMENT 9: Continues Operation for Alfa-amylase Production from E. coli
Homework
Week 12: EXPERIMENT 10: Continues Operation Analysis
Homework
Week 13: Continues Operation Analysis
Week 14: Experimental Discussion and Evaluation
Week 15*: -
Week 16*: Final Exam
Textbooks and materials:
Recommended readings: Doran, P. M., (2013). Bioprocess engineering principles, Elsevier Science

Mandenius, C. F. (2016). Bioreactors: design, operation and novel applications. John Wiley & Sons.

Saha, G., Barua, A., & Sinha, S. (2017). Bioreactors: Animal Cell Culture Control for Bioprocess Engineering. CRC Press.
  * Between 15th and 16th weeks is there a free week for students to prepare for final exam.
Assessment Up
Method of assessment Week number Weight (%)
Mid-terms: 0 0
Other in-term studies: 0 0
Project: 0 0
Homework: 2,3,4,5,6,7,9,10,11,12 60
Quiz: 0 0
Final exam: 16 40
  Total weight:
(%)
   Workload Up
Activity Duration (Hours per week) Total number of weeks Total hours in term
Courses (Face-to-face teaching): 3 12
Own studies outside class: 4 10
Practice, Recitation: 0 0
Homework: 0 0
Term project: 0 0
Term project presentation: 0 0
Quiz: 0 0
Own study for mid-term exam: 0 0
Mid-term: 0 0
Personal studies for final exam: 3 14
Final exam: 2 1
    Total workload:
    Total ECTS credits:
*
  * ECTS credit is calculated by dividing total workload by 25.
(1 ECTS = 25 work hours)
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