• Basic information
• Learning outcomes
• Contents
• Assessment
• Workload

Learning outcomes

Upon successful completion of this course, students will be able to:

1. To explain construction of antbody and antibody engineeering via recombinant DNA technologies

   Contribution to Program Outcomes

   1. Define, Chemical, Biological, Radiological and Nuclear (cbrn) concepts in a specialized way and apply them in CBRN defense
   2. Analyze critically and evaluate his/her findings and those of others
   3. Asses risks of natura lor human made CBRN threats
   4. Develop an awareness of continuous learning in relation with modern technology
   5. Be aware of issues relating to the rights of other researchers and of research subjects e.g. confidentiality, attribution, copyright, ethics, malpractice, ownership of data

   Method of assessment

   1. Written exam
2. To understand new antibody engineering application

   Contribution to Program Outcomes

   1. Define, Chemical, Biological, Radiological and Nuclear (cbrn) concepts in a specialized way and apply them in CBRN defense
   2. Write progress reports clearly on the basis of published documents, thesis, etc
   3. Asses risks of natura lor human made CBRN threats
   4. Find out new methods to improve his/her knowledge
   5. Effectively express his/her research ideas and findings both orally and in writing

   Method of assessment

   1. Written exam
3. They learn the mechanism of action of antibody structures.

   Contribution to Program Outcomes

   1. Define, Chemical, Biological, Radiological and Nuclear (cbrn) concepts in a specialized way and apply them in CBRN defense
   2. Analyze critically and evaluate his/her findings and those of others
   3. Develop an awareness of continuous learning in relation with modern technology
   4. Effectively express his/her research ideas and findings both orally and in writing
   5. Be aware of issues relating to the rights of other researchers and of research subjects e.g. confidentiality, attribution, copyright, ethics, malpractice, ownership of data

   Method of assessment

   1. Written exam

Contents
Week 1:	Immune system
Week 2:	Immunoglobulin engcoding genes
Week 3:	Steucture of immunoglobulins
Week 4:	Antibody libraries
Week 5:	Hybridoma and phages display methods
Week 6:	Midterm exam
Week 7:	Recombinant antibody technology
Week 8:	Production of immunoglobulins
Week 9:	Fragments of immunoglobulins
Week 10:	Prufication of Immunoglobulins
Week 11:	Characterization of immunoglobulins
Week 12:	Antibody implementation
Week 13:	Production of antibodies
Week 14:	Biopharmaceutics
Week 15*:	General overview
Week 16*:	Final exam
Textbooks and materials:	Antibody engineering/ Roland Kontermann and Stefan Dübel
Recommended readings:	Berrin Erdağ, Bertan Koray Balcıoğlu, Antikor Mühendisliği ve Uygulamaları, Bölüm 10, sayfa 215-, Güncel Biyoteknoloji ve Uygulamaları, Editörler Munis Dündar ve Haydar Bağış, MGrup Matbaacılık, Kayseri 2017. ISBN 978-605-67442-0-4. 2. Antibody Engineering, Methods and protocols, Benny K. C. lo. Winter G, Griffiths AD, Hawkins RE, Hoogenboom HR, Making Antibodies by Phage Display Technology. Annual Review of Immunology, 1994. 12: p. 433–55. Zola H, Thomas D, Lopez A, Monoclonal Antibodies: Therapeutic Uses. 2013.
	* Between 15th and 16th weeks is there a free week for students to prepare for final exam.

Assessment
Method of assessment	Week number	Weight (%)
Mid-terms:	6	35
Other in-term studies:		0
Project:		0
Homework:	10	15
Quiz:		0
Final exam:	16	50
	Total weight:	(%)

Workload
Activity	Duration (Hours per week)	Total number of weeks	Total hours in term
Courses (Face-to-face teaching):	3	14
Own studies outside class:	3	14
Practice, Recitation:	0	0
Homework:	6	10
Term project:	0	0
Term project presentation:	0	0
Quiz:	0	0
Own study for mid-term exam:	15	1
Mid-term:	1	1
Personal studies for final exam:	20	1
Final exam:	2	1
		Total workload:
		Total ECTS credits:	*
	* ECTS credit is calculated by dividing total workload by 25. (1 ECTS = 25 work hours)