• Basic information
• Learning outcomes
• Contents
• Assessment
• Workload

Learning outcomes

Upon successful completion of this course, students will be able to:

1. Learning the importance and usage of antibodies for different purposes in biodefense

   Contribution to Program Outcomes

   1. Define, Chemical, Biological, Radiological and Nuclear (cbrn) concepts in a specialized way and apply them in CBRN defense
   2. Understand relevant research methodologies and techniques and their appropriate application within his/her research field
   3. Analyze critically and evaluate his/her findings and those of others
   4. Summarize, document, report and reflect on progress
   5. Work effectively in multi-disciplinary research teams
   6. Asses risks of natura lor human made CBRN threats
   7. Develop an awareness of continuous learning in relation with modern technology
   8. Find out new methods to improve his/her knowledge
   9. Effectively express his/her research ideas and findings both orally and in writing
   10. Support his/her ideas with various arguments and present them clearly to a range of audience, formally and informally through a variety of techniques
   11. Be aware of issues relating to the rights of other researchers and of research subjects e.g. confidentiality, attribution, copyright, ethics, malpractice, ownership of data
   12. Apply of chemical, biological, radiological and nuclear concepts to individual, social, economic, technological and ethical aspects

   Method of assessment

   1. Written exam
   2. Homework assignment
2. Learn the development and production techniques of antibodies required for biodefence and apply basic cell culture methods.

   Contribution to Program Outcomes

   1. Define, Chemical, Biological, Radiological and Nuclear (cbrn) concepts in a specialized way and apply them in CBRN defense
   2. Understand relevant research methodologies and techniques and their appropriate application within his/her research field
   3. Analyze critically and evaluate his/her findings and those of others
   4. Summarize, document, report and reflect on progress
   5. Work effectively in multi-disciplinary research teams
   6. Asses risks of natura lor human made CBRN threats
   7. Develop an awareness of continuous learning in relation with modern technology
   8. Find out new methods to improve his/her knowledge
   9. Effectively express his/her research ideas and findings both orally and in writing
   10. Support his/her ideas with various arguments and present them clearly to a range of audience, formally and informally through a variety of techniques
   11. Be aware of issues relating to the rights of other researchers and of research subjects e.g. confidentiality, attribution, copyright, ethics, malpractice, ownership of data
   12. Understand relevant health and safety issues and demonstrate responsible working practices

   Method of assessment

   1. Written exam
   2. Homework assignment
3. Learn critical parameters to be monitored in large scale production of antibodies.

   Contribution to Program Outcomes

   1. Define, Chemical, Biological, Radiological and Nuclear (cbrn) concepts in a specialized way and apply them in CBRN defense
   2. Understand relevant research methodologies and techniques and their appropriate application within his/her research field
   3. Analyze critically and evaluate his/her findings and those of others
   4. Work effectively in multi-disciplinary research teams
   5. Asses risks of natura lor human made CBRN threats
   6. Develop an awareness of continuous learning in relation with modern technology
   7. Find out new methods to improve his/her knowledge
   8. Effectively express his/her research ideas and findings both orally and in writing
   9. Support his/her ideas with various arguments and present them clearly to a range of audience, formally and informally through a variety of techniques
   10. Be aware of issues relating to the rights of other researchers and of research subjects e.g. confidentiality, attribution, copyright, ethics, malpractice, ownership of data
   11. Understand relevant health and safety issues and demonstrate responsible working practices

   Method of assessment

   1. Written exam
   2. Homework assignment

Contents
Week 1:	Introduction – The Importance of Antibodies in Biodefence (Preventive, Protective, Therapeutic, Diagnostic)
Week 2:	Antibody Structures, Properties and Functions
Week 3:	Medical Protective and Therapeutic Antibodies
Week 4:	Antibodies for Diagnosis and Detection
Week 5:	Production of Antibodies by Cell Culture Techniques: Antibody Development with Hybridoma Technology (Rodent Antibodies)
Week 6:	Mid Term Exam
Week 7:	Antibody Development with Recombinant Technology (Human Antibodies)
Week 8:	Cell Culture Techniques
Week 9:	Cell Culture Techniques (Laboratory Applied)
Week 10:	Large Scale Antibody Production and Cell Culture Bioreactors
Week 11:	Culture Conditions in Bioreactors Upstream Processes
Week 12:	Kinetics of animal cell culture processes: Cell Kinetics in Batch Cultures Cell Kinetics in Continuous Cultures
Week 13:	Antibody Purification Downstream Processes
Week 14:	Product (Antibody) Characterization and Specifications
Week 15*:	Serum-free Medium for Monoclonal Antibody Production
Week 16*:	Final Exam
Textbooks and materials:	Basic Methods in Antibody Production and Characterization, CRC Press, NewYork, 2009
Recommended readings:	Basic Methods in Antibody Production and Characterization, CRC Press, NewYork, 2009
	* Between 15th and 16th weeks is there a free week for students to prepare for final exam.

Assessment
Method of assessment	Week number	Weight (%)
Mid-terms:	6	40
Other in-term studies:		0
Project:		0
Homework:		0
Quiz:		0
Final exam:	16	60
	Total weight:	(%)

Workload
Activity	Duration (Hours per week)	Total number of weeks	Total hours in term
Courses (Face-to-face teaching):	3	14
Own studies outside class:	7	14
Practice, Recitation:	0	0
Homework:	0	0
Term project:	1	1
Term project presentation:	1	1
Quiz:	0	0
Own study for mid-term exam:	20	1
Mid-term:	2	1
Personal studies for final exam:	20	1
Final exam:	2	1
		Total workload:
		Total ECTS credits:	*
	* ECTS credit is calculated by dividing total workload by 25. (1 ECTS = 25 work hours)