Syllabus ( CBRN 545 )
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Basic information
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| Course title: |
Recombinant DNA Technology |
| Course code: |
CBRN 545 |
| Lecturer: |
Prof. Dr. Uygar Halis TAZEBAY
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| ECTS credits: |
7.5 |
| GTU credits: |
3 (3+0+0) |
| Year, Semester: |
2018, Fall |
| Level of course: |
Second Cycle (Master's) |
| Type of course: |
Area Elective
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| Language of instruction: |
Turkish
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| Mode of delivery: |
Face to face
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| Pre- and co-requisites: |
None |
| Professional practice: |
No |
| Purpose of the course: |
The objective of the course is to give information about basic methods in recombinant DNA technology and the application areas of possible recombinant products which can be developed with these techniques. |
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Learning outcomes
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Upon successful completion of this course, students will be able to:
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Learn basic methods on recombinant DNA technology
Contribution to Program Outcomes
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Understand relevant research methodologies and techniques and their appropriate application within his/her research field
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Question and find out innovative approaches
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Work effectively in multi-disciplinary research teams
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Asses risks of natura lor human made CBRN threats
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Effectively express his/her research ideas and findings both orally and in writing
Method of assessment
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Written exam
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Seminar/presentation
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Obtain information on the use of recombinant microorganisms and / or productst against biological pathogens and pollutants.
Contribution to Program Outcomes
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Define, Chemical, Biological, Radiological and Nuclear (cbrn) concepts in a specialized way and apply them in CBRN defense
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Understand relevant research methodologies and techniques and their appropriate application within his/her research field
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Analyze critically and evaluate his/her findings and those of others
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Work effectively in multi-disciplinary research teams
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Develop stragies and/or product in order decicease risks of national and intenational CBRN threats
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Develop an awareness of continuous learning in relation with modern technology
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Support his/her ideas with various arguments and present them clearly to a range of audience, formally and informally through a variety of techniques
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Be aware of issues relating to the rights of other researchers and of research subjects e.g. confidentiality, attribution, copyright, ethics, malpractice, ownership of data
Method of assessment
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Written exam
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Homework assignment
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Learn about current methods of recombinant DNA technology
Contribution to Program Outcomes
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Define, Chemical, Biological, Radiological and Nuclear (cbrn) concepts in a specialized way and apply them in CBRN defense
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Understand relevant research methodologies and techniques and their appropriate application within his/her research field
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Analyze critically and evaluate his/her findings and those of others
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Develop stragies and/or product in order decicease risks of national and intenational CBRN threats
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Find out new methods to improve his/her knowledge
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Effectively express his/her research ideas and findings both orally and in writing
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Be aware of issues relating to the rights of other researchers and of research subjects e.g. confidentiality, attribution, copyright, ethics, malpractice, ownership of data
Method of assessment
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Written exam
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Homework assignment
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Contents
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| Week 1: |
A brief history of recombinant DNA technology |
| Week 2: |
Isolation and quantification of DNA/RNA |
| Week 3: |
Cloning vectors |
| Week 4: |
Enzymes used in molecular cloning |
| Week 5: |
Primer design for molecular cloning |
| Week 6: |
Polymerase chain reaction |
| Week 7: |
Ligation and transformation |
| Week 8: |
Mid-term exam |
| Week 9: |
Isolation of recombinant vectors |
| Week 10: |
DNA sequencing |
| Week 11: |
Expression of cloned genes |
| Week 12: |
Site directed mutagenesis of cloned DNA |
| Week 13: |
Analysis of recombinant proteins |
| Week 14: |
Potential application areas of recombinant products |
| Week 15*: |
Student presentations |
| Week 16*: |
Final exam |
| Textbooks and materials: |
1. Molecular Cloning: A Laboratory Manual, 3rd ed., Vols 1,2 and 3, J.F. Sambrook and D.W. Russell, Cold Spring Harbor Laboratory Press, 2001.
2. Gene Cloning and DNA Analysis, 6th ed., T.A. Brown, Wiley and Blackwell, 2010.
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| Recommended readings: |
Gene Cloning and DNA Analysis, 6th ed., T.A. Brown, Wiley and Blackwell, 2010. |
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* Between 15th and 16th weeks is there a free week for students to prepare for final exam.
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Assessment
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| Method of assessment |
Week number |
Weight (%) |
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| Mid-terms: |
8 |
35 |
| Other in-term studies: |
0 |
0 |
| Project: |
0 |
0 |
| Homework: |
15 |
15 |
| Quiz: |
0 |
50 |
| Final exam: |
16 |
0 |
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Total weight: |
(%) |
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Workload
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| Activity |
Duration (Hours per week) |
Total number of weeks |
Total hours in term |
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| Courses (Face-to-face teaching): |
3 |
14 |
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| Own studies outside class: |
6 |
14 |
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| Practice, Recitation: |
0 |
0 |
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| Homework: |
0 |
0 |
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| Term project: |
15 |
1 |
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| Term project presentation: |
1 |
1 |
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| Quiz: |
0 |
0 |
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| Own study for mid-term exam: |
20 |
1 |
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| Mid-term: |
2 |
1 |
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| Personal studies for final exam: |
20 |
1 |
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| Final exam: |
2 |
1 |
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Total workload: |
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Total ECTS credits: |
* |
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* ECTS credit is calculated by dividing total workload by 25.
(1 ECTS = 25 work hours)
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