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Syllabus ( MBG 223 )


   Basic information
Course title: Genetics Laboratory
Course code: MBG 223
Lecturer: Assist. Prof. Abu Musa Md Talimur REZA
ECTS credits: 3
GTU credits: 1 (0+4+0)
Year, Semester: 2, Fall
Level of course: First Cycle (Undergraduate)
Type of course: Compulsory
Language of instruction: English
Mode of delivery: Face to face , Lab work
Pre- and co-requisites: YES
Professional practice: No
Purpose of the course: Aim of this course is: to introduce students, some basic laboratory observations using plant, animal and human tissue materials.
   Learning outcomes Up

Upon successful completion of this course, students will be able to:

  1. Make chromosomal mutations and polyploidy using plant material and define them on microscope.

    Contribution to Program Outcomes

    1. To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
    2. To be able to explain the genetic information flow in organisms and populations.
    3. To be able to define life forms and their relationship with their environment.
    4. To be able to work individually, make independent decisions and participate actively in multidisciplinary group studies.

    Method of assessment

    1. Homework assignment
  2. İnvestigating the phenotypic characters in human and Drosophila. Comparing the difference between dominance and recessiveness.

    Contribution to Program Outcomes

    1. To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
    2. To be able to explain the genetic information flow in organisms and populations.
    3. To be able to define life forms and their relationship with their environment.

    Method of assessment

    1. Written exam
  3. enable to prepare and analyse the karyotypes

    Contribution to Program Outcomes

    1. To be able to define general concepts and problems related to Molecular Biology and Genetics and to produce solutions.
    2. To be able to define life forms and their relationship with their environment.
    3. To be able to work individually, make independent decisions and participate actively in multidisciplinary group studies.

    Method of assessment

    1. Written exam
   Contents Up
Week 1: Introduction to the model orgamisms for genetics laboratory works: yeast model, insect model, worm model, fish model (No laboratory work).
Week 2: Husbandry of model organism: preparation of medium, culturing, handling and maintaining.
Week 3: Observation of morphology, life cycle, sex and phenotype of the model organism.
Week 4: Mutant phenotype analysis in model organism.
Week 5: Observations of mitosis on plant root tips.
Week 6: Observations of X chromosome (Barr Body) in human.
Week 7: MID-TERM EXAM
Week 8: Constructing of karyotype using human chromosome spreads.
Week 9: Inducing chromosomal mutations and poliploidy in plants. Observing some chromosome and cell division
abnormalities on microscope.
Week 10: Observation of nucleolus organizing region (NOR) using mammalian cells
Week 11: A population genetics study using finger print ridge patterns in class population.
Week 12: Meiotic observations using plant pollen mother cells.
Week 13: Pedigree analysis and solving problems.
Week 14: Last day to submit the lab note
Week 15*: Preparation week for the final exam
Week 16*: FINAL EXAM
Textbooks and materials: 1- Genetics Laboratory Investigations, T. L Mertens and R. L. Hammersmith ,2000
2- Introduction to Genetic Analysis, J.F. Griffiths S. R. Wessler, 2007

Recommended readings: Cancer Cytogenetics: Chromosomal and Molecular Genetic Abberations of Tumor Cells
Plant Cytogenetics: Genome Structure and Chromosome Function (Plant Genetics and Genomics: Crops and Models)

  * Between 15th and 16th weeks is there a free week for students to prepare for final exam.
Assessment Up
Method of assessment Week number Weight (%)
Mid-terms: 8 30
Other in-term studies: 0
Project: 0
Homework: 15 20
Quiz: 0
Final exam: 16 50
  Total weight:
(%)
   Workload Up
Activity Duration (Hours per week) Total number of weeks Total hours in term
Courses (Face-to-face teaching): 3 15
Own studies outside class: 0 0
Practice, Recitation: 0 0
Homework: 6 1
Term project: 0 0
Term project presentation: 0 0
Quiz: 0 0
Own study for mid-term exam: 7 2
Mid-term: 2 1
Personal studies for final exam: 10 1
Final exam: 2 1
    Total workload:
    Total ECTS credits:
*
  * ECTS credit is calculated by dividing total workload by 25.
(1 ECTS = 25 work hours)
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