Syllabus ( MBG 527 )
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Basic information
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| Course title: |
Molecular Language of Organelles |
| Course code: |
MBG 527 |
| Lecturer: |
Prof. Dr. Uygar Halis TAZEBAY
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| ECTS credits: |
7,5 |
| GTU credits: |
3 (3+0+0) |
| Year, Semester: |
2025, Fall |
| Level of course: |
Second Cycle (Master's) |
| Type of course: |
Departmental Elective
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| Language of instruction: |
English
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| Mode of delivery: |
Face to face
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| Pre- and co-requisites: |
"none" |
| Professional practice: |
No |
| Purpose of the course: |
The main objective of this course is to understand the membraneless organelles formed by phase separation in cells, their formation mechanisms, structure and organization. Also, to learn the concept and methodology of phase separation and its relation with diseases. |
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Learning outcomes
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Upon successful completion of this course, students will be able to:
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Grasp basic and alternative functional mechanisms of membrane organelles in the cell.
Contribution to Program Outcomes
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Define and manipulate advanced concepts of Biology
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Critically review the literature pertaining to his/her research projects, and connect the earlier literature to his/her own results,
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Ability to work independently and take responsibility
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Acquire scientific knowledge and work independently,
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Find out new methods to improve his/her knowledge.
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Understand the applications and basic principles of new instrumentation and/or software vital to his/her thesis projects.
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Effectively express his/her research ideas and findings both orally and in writing
Method of assessment
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Written exam
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Seminar/presentation
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Grasp the relationship between membraneless organelles and human health.
Contribution to Program Outcomes
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Define and manipulate advanced concepts of Biology
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Critically review the literature pertaining to his/her research projects, and connect the earlier literature to his/her own results,
-
Ability to work independently and take responsibility
-
Acquire scientific knowledge and work independently,
-
Find out new methods to improve his/her knowledge.
-
Understand the applications and basic principles of new instrumentation and/or software vital to his/her thesis projects.
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Effectively express his/her research ideas and findings both orally and in writing
Method of assessment
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Written exam
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Seminar/presentation
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Understand membraneless organelles
Contribution to Program Outcomes
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Define and manipulate advanced concepts of Biology
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Critically review the literature pertaining to his/her research projects, and connect the earlier literature to his/her own results,
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Ability to work independently and take responsibility
-
Acquire scientific knowledge and work independently,
-
Find out new methods to improve his/her knowledge.
-
Understand the applications and basic principles of new instrumentation and/or software vital to his/her thesis projects.
-
Effectively express his/her research ideas and findings both orally and in writing
Method of assessment
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Written exam
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Seminar/presentation
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Contents
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| Week 1: |
Introduction
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| Week 2: |
Regulation of Biomolecular Condensate |
| Week 3: |
Molecular Language of Membraneless nuclear organelles-1 (Nuclear speckles, paraspecles, nucleous) |
| Week 4: |
Molecular Language of Membraneless nuclear organelles-1 Nuclear stress body, Cajal body, germ granule, PML-body) |
| Week 5: |
Ribosomal movements |
| Week 6: |
Processing bodies-I |
| Week 7: |
Mid-term exam-Processing bodies-II |
| Week 8: |
The role of Stress granules |
| Week 9: |
Quality control of membraneless organelles
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| Week 10: |
LLPS in microbial cells |
| Week 11: |
Techniques and methods used to study LLPS
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| Week 12: |
Cellular studies of liquid-liquid phase separation |
| Week 13: |
Phase separation in human health
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| Week 14: |
Student project presentations |
| Week 15*: |
- |
| Week 16*: |
FİNAL EXAM
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| Textbooks and materials: |
1. Functionally Relevant Macromolecular Interactions of Disordered Proteins;ISBN 978-3-03936-522-7 (2020)
2. Intrinsically Disordered Proteins and Chronic Diseases; ISBN978-3-0365-1263-1(2021).
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| Recommended readings: |
Alberti S, Carra S. Quality Control of Membraneless Organelles. J Mol Biol. 2018 Nov 2;430(23):4711-4729. doi: 10.1016/j.jmb.2018.05.013. Epub 2018 May 11. PMID: 29758260.
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* Between 15th and 16th weeks is there a free week for students to prepare for final exam.
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Assessment
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| Method of assessment |
Week number |
Weight (%) |
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| Mid-terms: |
7 |
30 |
| Other in-term studies: |
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0 |
| Project: |
14 |
20 |
| Homework: |
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0 |
| Quiz: |
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0 |
| Final exam: |
16 |
50 |
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Total weight: |
(%) |
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Workload
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| Activity |
Duration (Hours per week) |
Total number of weeks |
Total hours in term |
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| Courses (Face-to-face teaching): |
3 |
14 |
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| Own studies outside class: |
4 |
14 |
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| Practice, Recitation: |
0 |
0 |
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| Homework: |
0 |
0 |
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| Term project: |
0 |
0 |
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| Term project presentation: |
20 |
1 |
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| Quiz: |
0 |
0 |
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| Own study for mid-term exam: |
4 |
7 |
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| Mid-term: |
2 |
1 |
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| Personal studies for final exam: |
4 |
8 |
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| Final exam: |
3 |
1 |
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Total workload: |
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Total ECTS credits: |
* |
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* ECTS credit is calculated by dividing total workload by 25.
(1 ECTS = 25 work hours)
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