• Basic information
• Learning outcomes
• Contents
• Assessment
• Workload

Learning outcomes

Upon successful completion of this course, students will be able to:

1. Assess prokaryotic gene structure and function.

   Contribution to Program Outcomes

   1. Define and manipulate advanced concepts of Biology
   2. Identify structure-function relationships in cells and organisms
   3. Acquire scientific knowledge and work independently,
   4. Find out new methods to improve his/her knowledge.

   Method of assessment

   1. Written exam
2. Gain knowledge about the mechanisms of DNA synthesis, transcription and translation in prokaryotes.

   Contribution to Program Outcomes

   1. Define and manipulate advanced concepts of Biology
   2. Acquire scientific knowledge and work independently,
   3. Find out new methods to improve his/her knowledge.

   Method of assessment

   1. Written exam
   2. Homework assignment
3. Tell how the gene expression is regulated in prokaryotes.

   Contribution to Program Outcomes

   1. Define and manipulate advanced concepts of Biology
   2. Acquire scientific knowledge and work independently,
   3. Find out new methods to improve his/her knowledge.

   Method of assessment

   1. Written exam
   2. Homework assignment

Contents
Week 1:	General view of the prokaryotic cell, cell growth and cell cycle
Week 2:	*DNA Structure and Genetic Information *Supercoiling and histone like proteins *Chromosomes and Other Genetic Elements *Analytic methods in DNA and RNA research
Week 3:	*Mechanisms of DNA replication in prokaryotes *Short overview of eukaryotic DNA replication
Week 4:	*Mechanisms of Transcription in prokaryotes *Short overview of eukaryotic Transcription
Week 5:	*Mechanisms of translation in prokaryotes
Week 6:	*Short overview of eukaryotic translation *Genetic code *Protein Folding and Secretion *Molecular chaperones
Week 7:	*REGULATION *DNA-Binding Proteins and Transcriptional Regulation *‘Negative control’ versus ‘positive control’
Week 8:	Midterm exam and evaluation
Week 9:	*Catabolite Repression *Sensing and Signal Transduction *Quorum Sensing *Heat Shock Proteins *Stringent Response
Week 10:	*Alternative sigma factors *RNA-Based Regulation *Attenuation
Week 11:	*Regulation of translation and post-translational modifications
Week 12:	*Methods for Manipulating DNA *Essentials of Molecular Cloning *Molecular Methods for Mutagenesis
Week 13:	*Gene Fusions and Reporter Genes *Cloning Vectors *Hosts for Cloning Vectors *Shuttle Vectors
Week 14:	*Expression Vectors *Regulation of Transcription from Expression Vectors
Week 15*:	*Expressing Genes in Bacteria
Week 16*:	Final exam
Textbooks and materials:	PDF files covered in the lecture
Recommended readings:	1. Watson, J.D., et al. Molecular Biology of the Gene. 7. ed. The Benjamin/Cummings Pub. Co., Menlo Park, California. 2. Madigan, M.T., Martinko, J.M., Bender, K.S., Buckley, D. H., Stahl, D.A. 15th Ed. Brock Biology of Microorganisims. Pearson Education Inc. San Francisco 3. Alberts, B., et al. Molecular Biology of the Cell. 3. ed. Garland Pub., New York, 1994. 4. Lewin, B. Genes VII. Oxford University Pres, Oxford, 2000. 5. Biology of the Prokaryotes, Lengeler, J., Schlegal, H. (Eds), 1999, Blackwell Publishing, Stutgart, Germany. 6. The World of the Cell, Becker, W.M., Kleinsmith, L.J., and Hardin, J.,2000, Addison Wesley Publishing Company, Masachusetts,U.S.A. 7. Wagner, R. Transcription Regulation in Prokaryotes. Oxford University Press, 2000.
	* Between 15th and 16th weeks is there a free week for students to prepare for final exam.

Assessment
Method of assessment	Week number	Weight (%)
Mid-terms:	8	40
Other in-term studies:		0
Project:		0
Homework:		0
Quiz:		0
Final exam:	16	60
	Total weight:	(%)

Workload
Activity	Duration (Hours per week)	Total number of weeks	Total hours in term
Courses (Face-to-face teaching):	3	15
Own studies outside class:	4	15
Practice, Recitation:	0	0
Homework:	0	0
Term project:	0	0
Term project presentation:	0	0
Quiz:	0	0
Own study for mid-term exam:	20	2
Mid-term:	2	1
Personal studies for final exam:	20	2
Final exam:	2	1
		Total workload:
		Total ECTS credits:	*
	* ECTS credit is calculated by dividing total workload by 25. (1 ECTS = 25 work hours)