ECTS @ IUE ECTS @ IUE ECTS @ IUE ECTS @ IUE ECTS @ IUE ECTS @ IUE ECTS @ IUE

Syllabus ( MBG 643 )


   Basic information
Course title: Gene And Protein Engineering
Course code: MBG 643
Lecturer: Assist. Prof. Saliha İŞSEVER ÖZTÜRK
ECTS credits: 7.5
GTU credits: 3 (3+0+0)
Year, Semester: 1/2, Fall and Spring
Level of course: Second Cycle (Master's)
Type of course: Area Elective
Language of instruction: Turkish
Mode of delivery: Face to face
Pre- and co-requisites: None
Professional practice: No
Purpose of the course: The objective of this course is to get the students to comprehend the principles of the main techniques used in genetic and protein engineering /molecular biotechnology and to introduce the recent technologies.
   Learning outcomes Up

Upon successful completion of this course, students will be able to:

  1. Recognise the principles of fundemental techniques used in genetic and protein engineering / molecular biotechnology

    Contribution to Program Outcomes

    1. Define and manipulate advanced concepts of Biology
    2. Critically review the literature pertaining to his/her research projects, and connect the earlier literature to his/her own results,
    3. Know and apply the techniques used in gene and protein engineering / microbial identification / enzyme technologies / mammalian cell culture and plant tissue culture manipulations.
    4. Ability to work independently and take responsibility
    5. Find out new methods to improve his/her knowledge.
    6. Understand the applications and basic principles of new instrumentation and/or software vital to his/her thesis projects.
    7. Understand the importance of identification, utilization and maintenance of the biological diversity in the country.

    Method of assessment

    1. Written exam
  2. Identify recent technologies used in these areas.

    Contribution to Program Outcomes

    1. Define and manipulate advanced concepts of Biology
    2. Critically review the literature pertaining to his/her research projects, and connect the earlier literature to his/her own results,
    3. Know and apply the techniques used in gene and protein engineering / microbial identification / enzyme technologies / mammalian cell culture and plant tissue culture manipulations.
    4. Ability to work independently and take responsibility
    5. Understand the applications and basic principles of new instrumentation and/or software vital to his/her thesis projects.

    Method of assessment

    1. Written exam
    2. Seminar/presentation
    3. Term paper
  3. Do detailed literature research on a current topic of molecular biology.

    Contribution to Program Outcomes

    1. Define and manipulate advanced concepts of Biology
    2. Critically review the literature pertaining to his/her research projects, and connect the earlier literature to his/her own results,
    3. Acquire scientific knowledge and work independently,
    4. Find out new methods to improve his/her knowledge.
    5. Understand the applications and basic principles of new instrumentation and/or software vital to his/her thesis projects.
    6. Effectively express his/her research ideas and findings both orally and in writing

    Method of assessment

    1. Seminar/presentation
    2. Term paper
   Contents Up
Week 1: r-DNA technologies, short history of the genetic and protein engineering












Week 2: Isolation of the DNA and RNA, electrophoresis of the nucleic acids
Week 3: Hybridization of the nucleic acids, Southern and Northern Blot analysis
Week 4: The use of enzymes in the modification of nucleic acids
Week 5: Restriction enzymes
Week 6: Polimerase chain reaction and its applications
Week 7: DNA sequencing
Week 8: Midterm exam
Week 9: Recombinant protein expression, expression systems (vectors and hosts), homologue and heterologue gene expression aproaches
Week 10: Genom and proteom analysis, functional geneomics, techniques to determine protein-protein and protein-DNA interactions
Week 11: Protein isolation and purification, electrophoretic and chromatographic techniques, Western Blot analysis
Week 12: Aproaches used in protein engineering: rational design and site directed mutagenesis, directed evolution and library creation
Week 13: Molecular diagnostics in medicine, techniques used in drug research and industry
Week 14: Molecular based techniques used in determination and also characterization of the biodiversity
Week 15*: General review
Week 16*: Final exam
Textbooks and materials:
Recommended readings: 1. Wink, M. (Ed), An Introduction to Molecular Biotechnology, Wiley-VCH, Weinheim, Germany, 2006.
2. Cleland, J.L. and Craik, C.S. (Eds), Protein Engineering: Principals and Practice, Wiley-Liss Publication, New York, USA, 1996,
3. Maniatis, T. et al. Molecular Cloning: A Laboratory Manual, Cold Spring Harbour Laboratory Press, Cold Spring Harbour, N.Y., 1982,
4. Arnold, F.H. and Georgiou, G. (Eds), Directed Evolution Library Creation, Humana Pres, New Jersey, USA, 2003,
5. Brakmann, S. And Schwienhorst, A., Evolutionary Methods in Biotechnology, Wiley-VCH, WeinheiKm, 2004.
  * Between 15th and 16th weeks is there a free week for students to prepare for final exam.
Assessment Up
Method of assessment Week number Weight (%)
Mid-terms: 8 40
Other in-term studies: 0
Project: 12 10
Homework: 0
Quiz: 0
Final exam: 16 50
  Total weight:
(%)
   Workload Up
Activity Duration (Hours per week) Total number of weeks Total hours in term
Courses (Face-to-face teaching): 3 14
Own studies outside class: 6 14
Practice, Recitation: 0 0
Homework: 0 0
Term project: 4 4
Term project presentation: 1 1
Quiz: 0 0
Own study for mid-term exam: 10 2
Mid-term: 2 1
Personal studies for final exam: 10 2
Final exam: 2 1
    Total workload:
    Total ECTS credits:
*
  * ECTS credit is calculated by dividing total workload by 25.
(1 ECTS = 25 work hours)
-->