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Contents
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Week 1: |
r-DNA technologies, short history of the genetic and protein engineering
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Week 2: |
Isolation of the DNA and RNA, electrophoresis of the nucleic acids |
Week 3: |
Hybridization of the nucleic acids, Southern and Northern Blot analysis |
Week 4: |
The use of enzymes in the modification of nucleic acids |
Week 5: |
Restriction enzymes |
Week 6: |
Polimerase chain reaction and its applications |
Week 7: |
DNA sequencing |
Week 8: |
Midterm exam |
Week 9: |
Recombinant protein expression, expression systems (vectors and hosts), homologue and heterologue gene expression aproaches |
Week 10: |
Genom and proteom analysis, functional geneomics, techniques to determine protein-protein and protein-DNA interactions |
Week 11: |
Protein isolation and purification, electrophoretic and chromatographic techniques, Western Blot analysis |
Week 12: |
Aproaches used in protein engineering: rational design and site directed mutagenesis, directed evolution and library creation
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Week 13: |
Molecular diagnostics in medicine, techniques used in drug research and industry |
Week 14: |
Molecular based techniques used in determination and also characterization of the biodiversity |
Week 15*: |
General review |
Week 16*: |
Final exam |
Textbooks and materials: |
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Recommended readings: |
1. Wink, M. (Ed), An Introduction to Molecular Biotechnology, Wiley-VCH, Weinheim, Germany, 2006. 2. Cleland, J.L. and Craik, C.S. (Eds), Protein Engineering: Principals and Practice, Wiley-Liss Publication, New York, USA, 1996, 3. Maniatis, T. et al. Molecular Cloning: A Laboratory Manual, Cold Spring Harbour Laboratory Press, Cold Spring Harbour, N.Y., 1982, 4. Arnold, F.H. and Georgiou, G. (Eds), Directed Evolution Library Creation, Humana Pres, New Jersey, USA, 2003, 5. Brakmann, S. And Schwienhorst, A., Evolutionary Methods in Biotechnology, Wiley-VCH, WeinheiKm, 2004. |
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* Between 15th and 16th weeks is there a free week for students to prepare for final exam.
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