• Basic information
• Learning outcomes
• Contents
• Assessment
• Workload

Learning outcomes

Upon successful completion of this course, students will be able to:

1. Grasp the usage of different microbial hosts for metabolic engineering.

   Contribution to Program Outcomes

   1. Define and manipulate advanced concepts of Biology in a specialized way
   2. Develop an awareness of continuous learning in relation with modern technology

   Method of assessment

   1. Seminar/presentation
2. Explain key methodologies in metabolic engineering and synthetic biology.

   Contribution to Program Outcomes

   1. Understand relevant research methodologies and techniques and their appropriate application within his/her research field,
   2. Find out new methods to improve his/her knowledge.

   Method of assessment

   1. Written exam
   2. Seminar/presentation
3. Grasp the role of engineered biological systems in achieving sustainability goals

   Contribution to Program Outcomes

   1. Define and manipulate advanced concepts of Biology in a specialized way
   2. Apply biological concepts to personal, social, economical, technological and ethical issues

   Method of assessment

   1. Written exam

Contents
Week 1:	The Importance of Synthetic Biology
Week 2:	Biological Parts, Modularity and Standardization
Week 3:	Construction of Synthetic Pathways
Week 4:	Applications of Synthetic Biology
Week 5:	Case-study_I (Synthetic microbial consortia)
Week 6:	Case study-II (iGEM competition, ethical concern about synthetic biology)
Week 7:	Midterm Exam-Microbial cell factories for Metabolic Engineering
Week 8:	Design of Metabolic Pathways
Week 9:	Regulation of Metabolic Pathways
Week 10:	Metabolic Engineering Strategies, focusing on genome editing tools like CRISPR and other advanced techniques
Week 11:	Applications of Metabolic Engineering: Biodegradation, Bioremediation, Biomass conversion
Week 12:	Applications of Metabolic Engineering: Biosynthesis of primary and secondary metabolites
Week 13:	Applications of Metabolic Engineering: Agriculture, Food technology
Week 14:	Applications of Metabolic Engineering: Agriculture, Food technology
Week 15*:	-
Week 16*:	Final Exam
Textbooks and materials:	Nielsen, J., Stephanopoulos, G. and Lee, S.Y. eds., 2021. Metabolic Engineering: Concepts and Applications. Volume 13a. Wiley-VCH. Zhao, H. and Zeng, A.P. eds., 2018. Synthetic biology-metabolic engineering. Springer International Publishing.
Recommended readings:	Kuldell, N., Bernstein, R., Ingram, K., & Hart, K. M. (2015). BioBuilder: Synthetic biology in the lab. O'Reilly. Baldwin, G. (2012). Synthetic biology: A primer. John Wiley & Sons. Church, G. M., & Regis, E. (2014). Regenesis: how synthetic biology will reinvent nature and ourselves. Basic Books. Stephanopoulos, G., Aristidou, A.A., Nielsen, J. (1998). Metabolic engineering: principles and methodologies. Carbonell, P. (2019). Metabolic Pathway Design. Springer.
	* Between 15th and 16th weeks is there a free week for students to prepare for final exam.

Assessment
Method of assessment	Week number	Weight (%)
Mid-terms:	7	30
Other in-term studies:	4, 6, 8, 10,12,13	30
Project:		0
Homework:		0
Quiz:		0
Final exam:	16	40
	Total weight:	(%)

Workload
Activity	Duration (Hours per week)	Total number of weeks	Total hours in term
Courses (Face-to-face teaching):	3	14
Own studies outside class:	4	14
Practice, Recitation:	0	0
Homework:	0	0
Term project:	3	6
Term project presentation:	1	6
Quiz:	0	0
Own study for mid-term exam:	4	6
Mid-term:	2	1
Personal studies for final exam:	5	7
Final exam:	2	1
		Total workload:
		Total ECTS credits:	*
	* ECTS credit is calculated by dividing total workload by 25. (1 ECTS = 25 work hours)