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Syllabus ( MBG 667 )


   Basic information
Course title: Plant Metabolic Engineering
Course code: MBG 667
Lecturer: Assoc. Prof. Dr. Fatma AYDINOĞLU
ECTS credits: 7.5
GTU credits: 3 (3+0+0)
Year, Semester: 1/2/3/4, Fall and Spring
Level of course: Second Cycle (Master's)
Type of course: Area Elective
Language of instruction: English
Mode of delivery: Face to face
Pre- and co-requisites: No
Professional practice: No
Purpose of the course: Grasping types, biosynthesis and regulation of plant secondary metabolites. Giving detail information about plant secondary metabolites' physiological effects and usage area. Becoming familiar with the genetic engineering techniques to improve the production of plant secondary metabolite by functional genomics and omics technologies approach.
   Learning outcomes Up

Upon successful completion of this course, students will be able to:

  1. Distinguish between plant natural secondary metabolites' types.

    Contribution to Program Outcomes

    1. Define and manipulate advanced concepts of Biology
    2. Develop an understanding of matter and energy in organisms

    Method of assessment

    1. Written exam
    2. Oral exam
  2. List the practical usage area of plant secondary metabolites in biotechnology and explain each one.

    Contribution to Program Outcomes

    1. Define and manipulate advanced concepts of Biology
    2. Know and apply the techniques used in gene and protein engineering / microbial identification / enzyme technologies / mammalian cell culture and plant tissue culture manipulations.

    Method of assessment

    1. Written exam
    2. Homework assignment
  3. Choose the suitable strategy and approach for identification of the whole plant secondary metabolites and only one specific metabolite among those metabolites.

    Contribution to Program Outcomes

    1. Define and manipulate advanced concepts of Biology
    2. Critically review the literature pertaining to his/her research projects, and connect the earlier literature to his/her own results,
    3. Identify structure-function relationships in cells and organisms
    4. Acquire scientific knowledge and work independently,
    5. Effectively express his/her research ideas and findings both orally and in writing

    Method of assessment

    1. Written exam
    2. Oral exam
    3. Homework assignment
  4. Identify Plant secondary metabolite biosynthesis and regulation.

    Contribution to Program Outcomes

    1. Define and manipulate advanced concepts of Biology

    Method of assessment

    1. Written exam
    2. Oral exam
    3. Homework assignment
   Contents Up
Week 1: Overview of Plant Metabolism, Carbon Metabolism
Week 2: Plant primary metabolites: Carbohydrates, Fatty acids, Amino acids, Nucleotids, Phytosterols, Phytohormones
Week 3: Plant secondary metabolites and Classification according to Phsiological effects, Chemical structure and Their occurence
Week 4: Classification of Plant secondary metabolites according to their biosynthetic structure
1- Terponoids: Metabolic engineering of Terponoids' biosynthesis, Synthesis of IPP, Prenyltransferase and terpene synthesis reactions, Modification of terponoid skeletons, Toward transgenic terponoid
Week 5: Classification of Plant secondary metabolites according to their biosynthetic structure
2- Alkoloids: Metabolic engineering of Alkoloids' biosynthesis, Biotechnological application of alkoloid biosynthesis research
Week 6: Classification of Plant secondary metabolites according to their biosynthetic structure
3- Phenylpropanoids/phenolic compounds: Metabolic engineering of Phenylpropanoid and phenylpropanoid-acetate pathway metabolites,
Week 7: Midterm exam
Week 8: Classification of Plant secondary metabolites according to their biosynthetic structure
3- Phenylpropanoids/phenolic compounds: Biosynthesis of lignans, lignins, and suberization
Week 9: Classification of Plant secondary metabolites according to their biosynthetic structure
3- Phenylpropanoids/phenolic compounds: Metabolic engineering of Flavonoid biosynthesis
Week 10: Classification of Plant secondary metabolites according to their biosynthetic structure
3- Phenylpropanoids/phenolic compounds: Coumarins, stilbens, strylpyrones and arylpyrones
Week 11: Classification of Plant secondary metabolites according to their biosynthetic structure
3- Phenylpropanoids/phenolic compounds: Metabolic engineering of phenylpropanoid production: Fibers, pigments, pharmaceuticals and flavoring agents production
Week 12: Functional genetics approaches in Plant Metobolic Engineering: Forward genetics and reverse genetics
Week 13: Omics Technologies in Plant Metobolic Engineering, Metabolomics
Week 14: The role of plant secondary metabolites in plant defense
Week 15*: Discussions of current papers related with the topics
Week 16*: Final exam
Textbooks and materials: Metabolic Engineering of Plant Secondary Metabolism, R. Verpoorte, A. Wilhelm Alfermann, Springer, 2000.
Biochemistry & Molecular Biology of Plants, Bob Buchanan, Wilhelm Gruissem, Russell Jones (Ed.), 2002.
Plant Biochemistry, Fourth Edition, Hans-Walter Heldt, Birgit Piechulla, 2010.
Recommended readings: Plant Secondary Metabolism Engineering, Methods and Applications Vol. 643, Arthur Germano Fett-Neto (Ed), Humana Press, 2010.
Plant Physiology, Fifth Edition, Lincoln Taiz, 2010.
  * Between 15th and 16th weeks is there a free week for students to prepare for final exam.
Assessment Up
Method of assessment Week number Weight (%)
Mid-terms: 7 20
Other in-term studies: 2,4,6,8,10 20
Project: 0
Homework: 8,9,10,11,12,13,14 20
Quiz: 0
Final exam: 16 40
  Total weight:
(%)
   Workload Up
Activity Duration (Hours per week) Total number of weeks Total hours in term
Courses (Face-to-face teaching): 3 14
Own studies outside class: 5 14
Practice, Recitation: 0 0
Homework: 5 7
Term project: 0 0
Term project presentation: 0 0
Quiz: 0 0
Own study for mid-term exam: 15 1
Mid-term: 2 1
Personal studies for final exam: 20 1
Final exam: 2 1
    Total workload:
    Total ECTS credits:
*
  * ECTS credit is calculated by dividing total workload by 25.
(1 ECTS = 25 work hours)
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